AATK (NM_001080395) Human Tagged ORF Clone
Product Information
| Product Name | AATK (NM_001080395) Human Tagged ORF Clone |
| Cat.No | CDEP1005 |
| Description | AATK (Myc-DDK-tagged)-Human apoptosis-associated tyrosine kinase (AATK) |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | AATK |
| Synonyms | AATYK; AATYK1; LMR1; LMTK1; p35BP; PPP1R77 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Sequence Data | ORF Nucleotide Sequence (showhide) >RC219135 representing NM_001080395 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACGCAGAATGGGCCAAGGAGCTGGCTCTGCCCACCTGTCCCGCTGGCCTGGCAGGTGACGGTGCTGG ATGTGGCCTTTTTGCCTTTTCTAAAGGCCACATTTTCCAGCCCATTCAACCTTCCAGAGCCCTCTGAAGT GGCCACAGGCCCCATCAACCAGACCCGGCTGGCCCCCGGGCAGCAGGCTCTTAAGGAGTTCTGGGAGCTC CGGAGGTGGTGTGGCCACCAGGTGAAGGTCCAAGGCTGCTGGGGCAGATGGCGGTGGCAGGAGTTTGAGA ATGCGGAGGGGGACGAGTACGCAGCCGACCTGGCGCAGGGCTCCCCGGCCACGGCAGCACAGAACGGGCC CGACGTGTACGTCCTGCCACTCACGGAGGTCTCCTTGCCCATGGCCAAGCAGCCTGGGCGCTCAGTGCAG CTCCTCAAGTCCACAGACGTGGGCCGGCACAGCCTCCTGTACCTGAAGGAAATCGGCCGTGGCTGGTTCG GGAAGGTGTTCCTGGGGGAGGTGAACTCTGGCATCAGCAGTGCCCAGGTGGTGGTGAAGGAGCTGCAGGC TAGTGCCAGCGTGCAGGAGCAGATGCAGTTCCTGGAGGAGGTGCAGCCCTACAGGGCCCTGAAGCACAGC AACCTGCTCCAGTGCCTGGCCCAGTGCGCCGAGGTGACGCCCTACCTGCTGGTGATGGAGTTCTGCCCAC TGGGGGACCTCAAGGGCTACCTGCGGAGCTGCCGGGTGGCGGAGTCCATGGCTCCCGACCCCCGGACCCT GCAGCGCATGGCCTGTGAGGTGGCCTGTGGCGTCCTGCACCTTCATCGCAACAATTTCGTGCACAGCGAC CTGGCCCTGCGGAACTGCCTGCTCACGGCTGACCTGACGGTGAAGATTGGTGACTATGGCCTGGCTCACT GCAAGTACAGAGAGGACTACTTCGTGACTGCCGACCAGCTGTGGGTGCCTCTGCGCTGGATCGCGCCAGA GCTGGTGGACGAGGTGCATAGCAACCTGCTCGTCGTGGACCAGACCAAGAGCGGGAATGTGTGGTCCCTG GGCGTGACCATCTGGGAGCTCTTTGAGCTGGGCACGCAGCCCTATCCCCAGCACTCGGACCAGCAGGTGC TGGCGTACACGGTCCGGGAGCAGCAGCTCAAGCTGCCCAAGCCCCAGCTGCAGCTGACCCTGTCGGACCG CTGGTACGAGGTGATGCAGTTCTGCTGGCTGCAGCCCGAGCAGCGGCCCACAGCCGAGGAGGTGCACCTG CTGCTGTCCTACCTGTGTGCCAAGGGCGCCACCGAAGCAGAGGAGGAGTTTGAACGGCGCTGGCGCTCTC TGCGGCCCGGCGGGGGCGGCGTGGGGCCCGGGCCCGGTGCGGCGGGGCCCATGCTGGGCGGCGTGGTGGA GCTCGCCGCTGCCTCGTCCTTCCCGCTGCTGGAGCAGTTCGCGGGCGACGGCTTCCACGCGGACGGCGAC GACGTGCTGACGGTGACCGAGACCAGCCGAGGCCTCAATTTTGAGTACAAGTGGGAGGCGGGCCGCGGCG CGGAGGCCTTCCCGGCCACGCTGAGCCCTGGCCGCACCGCACGCCTCCAGGAGCTGTGCGCCCCCGACGG CGCGCCCCCGGGCGTGGTTCCGGTGCTCAGCGCGCACAGCCCGTCGCTGGGCAGCGAGTACTTCATCCGC CTAGAGGAGGCCGCACCCGCCGCCGGCCACGACCCTGACTGCGCCGGCTGCGCCCCCAGTCCACCTGCCA CCGCGGACCAGGACGACGACTCTGACGGCAGCACCGCCGCCTCGCTGGCCATGGAGCCGCTGCTGGGCCA CGGGCCACCCGTCGACGTCCCCTGGGGCCGCGGCGACCACTACCCTCGCAGAAGCTTGGCGCGGGACCCG CTCTGCCCCTCACGCTCTCCCTCGCCCTCGGCGGGGCCCCTGAGTCTGGCGGAGGGAGGAGCGGAGGATG CAGACTGGGGCGTGGCCGCCTTCTGTCCTGCCTTCTTCGAGGACCCACTGGGCACGTCCCCTTTGGGGAG CTCAGGGGCGCCCCCGCTGCCGCTGACTGGCGAGGATGAGCTAGAGGAGGTGGGAGCGCGGAGGGCCGCC CAGCGCGGGCACTGGCGCTCCAACGTGTCAGCCAACAACAACAGCGGCAGCCGCTGTCCAGAGTCCTGGG ACCCCGTCTCTGCGGGCGGCCACGCTGAGGGCTGCCCCAGTCCAAAGCAGACCCCACGGGCCTCCCCCGA GCCGGGGTACCCTGGAGAGCCTCTGCTTGGGCTCCAGGCAGCCTCTGCCCAGGAGCCAGGCTGCTGCCCC GGCCTCCCTCATCTATGCTCTGCCCAGGGCCTGGCACCTGCTCCCTGCCTGGTTACACCCTCCTGGACAG AGACAGCCAGTAGTGGGGGTGACCACCCGCAGGCAGAGCCCAAGCTTGCCACGGAGGCTGAGGGCACTAC CGGACCCCGCCTGCCCCTTCCTTCCGTCCCCTCCCCATCCCAGGAGGGAGCCCCACTTCCCTCGGAGGAG GCCAGTGCCCCCGACGCCCCTGATGCCCTGCCTGACTCTCCCACGCCTGCTACTGGTGGCGAGGTGTCTG CCATCAAGCTGGCTTCTGCCCTGAATGGCAGCAGCAGCTCTCCCGAGGTGGAGGCACCCAGCAGTGAGGA TGAGGACACGGCCGAGGCCACCTCAGGCATCTTCACCGACACGTCCAGCGACGGCCTGCAGGCCAGGAGG CCGGATGTGGTGCCAGCCTTCCGCTCTCTGCAGAAGCAGGTGGGGACCCCCGACTCCCTGGACTCCCTGG ACATCCCGTCCTCAGCCAGTGATGGTGGCTATGAGGTCTTCAGCCCGTCGGCCACTGGCCCCTCTGGAGG GCAGCCGCGAGCGCTGGACAGTGGCTATGACACCGAGAACTATGAGTCCCCTGAGTTTGTGCTCAAGGAG GCGCAGGAAGGGTGTGAGCCCCAGGCCTTTGCGGAGCTGGCCTCAGAGGGTGAGGGCCCCGGGCCCGAGA CACGGCTCTCCACCTCCCTCAGTGGCCTCAACGAGAAGAATCCCTACCGAGACTCTGCCTACTTCTCAGA CCTCGAGGCTGAGGCCGAGGCCACCTCAGGCCCAGAGAAGAAGTGCGGCGGGGACCGAGCCCCCGGGCCA GAGCTGGGCCTGCCGAGCACTGGGCAGCCGTCTGAGCAGGTCTGTCTCAGGCCTGGGGTTTCCGGGGAGG CACAAGGCTCTGGCCCCGGGGAGGTGCTGCCCCCACTGCTGCAGCTTGAAGGGTCCTCCCCAGAGCCCAG CACCTGCCCCTCGGGCCTGGTCCCAGAGCCTCCGGAGCCCCAAGGCCCAGCCAAGGTGCGGCCTGGGCCC AGCCCCAGCTGCTCCCAGTTTTTCCTGCTGACCCCGGTTCCGCTGAGATCAGAAGGCAACAGCTCTGAGT TCCAGGGGCCCCCAGGACTGTTGTCAGGGCCGGCCCCACAAAAGCGGATGGGGGGCCCAGGCACCCCCAG AGCCCCACTCCGCCTGGCTCTGCCCGGCCTCCCTGCGGCCTTGGAGGGCCGGCCGGAGGAGGAGGAGGAG GACAGTGAGGACAGCGACGAGTCTGACGAGGAGCTCCGCTGCTACAGCGTCCAGGAGCCTAGCGAGGACA GCGAAGAGGAGGCGCCGGCGGTGCCCGTGGTGGTGGCTGAGAGCCAGAGCGCGCGCAACCTGCGCAGCCT GCTCAAGATGCCCAGCCTGCTGTCCGAGACCTTCTGCGAGGACCTGGAACGCAAGAAGAAGGCCGTGTCC TTCTTCGACGACGTCACCGTCTACCTCTTTGACCAGGAAAGCCCCACCCGGGAGCTCGGGGAGCCCTTCC CGGGCGCCAAGGAATCGCCCCCTACGTCCCTTAGGGGGAGCCCCGGCTCTCCCAGCGCCCCCAACCGGCC GCAGCAGGCTGATGGCTCCCCAAATGGCTCCACAGCGGAAGAGGGTGGTGGGTTCGCGTGGGACGACGAC TTCCCGCTGATGACGGCCAAGGCAGCCTTCGCCATGGCCCTAGACCCGGCCGCACCCGCCCCGGCTGCGC CCACGCCCACGCCCGCTCCCTTCTCGCGCTTCACGGTGTCGCCCGCGCCCACGTCCCGCTTCTCCATCAC GCACGTGTCTGACTCGGACGCCGAGTCCAAGAGAGGACCTGAAGCTGGTGCCGGGGGTGAGAGTAAAGAG GCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC219135 representing NM_001080395 Red=Cloning site Green=Tags(s) MTQNGPRSWLCPPVPLAWQVTVLDVAFLPFLKATFSSPFNLPEPSEVATGPINQTRLAPGQQALKEFWEL RRWCGHQVKVQGCWGRWRWQEFENAEGDEYAADLAQGSPATAAQNGPDVYVLPLTEVSLPMAKQPGRSVQ LLKSTDVGRHSLLYLKEIGRGWFGKVFLGEVNSGISSAQVVVKELQASASVQEQMQFLEEVQPYRALKHS NLLQCLAQCAEVTPYLLVMEFCPLGDLKGYLRSCRVAESMAPDPRTLQRMACEVACGVLHLHRNNFVHSD LALRNCLLTADLTVKIGDYGLAHCKYREDYFVTADQLWVPLRWIAPELVDEVHSNLLVVDQTKSGNVWSL GVTIWELFELGTQPYPQHSDQQVLAYTVREQQLKLPKPQLQLTLSDRWYEVMQFCWLQPEQRPTAEEVHL LLSYLCAKGATEAEEEFERRWRSLRPGGGGVGPGPGAAGPMLGGVVELAAASSFPLLEQFAGDGFHADGD DVLTVTETSRGLNFEYKWEAGRGAEAFPATLSPGRTARLQELCAPDGAPPGVVPVLSAHSPSLGSEYFIR LEEAAPAAGHDPDCAGCAPSPPATADQDDDSDGSTAASLAMEPLLGHGPPVDVPWGRGDHYPRRSLARDP LCPSRSPSPSAGPLSLAEGGAEDADWGVAAFCPAFFEDPLGTSPLGSSGAPPLPLTGEDELEEVGARRAA QRGHWRSNVSANNNSGSRCPESWDPVSAGGHAEGCPSPKQTPRASPEPGYPGEPLLGLQAASAQEPGCCP GLPHLCSAQGLAPAPCLVTPSWTETASSGGDHPQAEPKLATEAEGTTGPRLPLPSVPSPSQEGAPLPSEE ASAPDAPDALPDSPTPATGGEVSAIKLASALNGSSSSPEVEAPSSEDEDTAEATSGIFTDTSSDGLQARR PDVVPAFRSLQKQVGTPDSLDSLDIPSSASDGGYEVFSPSATGPSGGQPRALDSGYDTENYESPEFVLKE AQEGCEPQAFAELASEGEGPGPETRLSTSLSGLNEKNPYRDSAYFSDLEAEAEATSGPEKKCGGDRAPGP ELGLPSTGQPSEQVCLRPGVSGEAQGSGPGEVLPPLLQLEGSSPEPSTCPSGLVPEPPEPQGPAKVRPGP SPSCSQFFLLTPVPLRSEGNSSEFQGPPGLLSGPAPQKRMGGPGTPRAPLRLALPGLPAALEGRPEEEEE DSEDSDESDEELRCYSVQEPSEDSEEEAPAVPVVVAESQSARNLRSLLKMPSLLSETFCEDLERKKKAVS FFDDVTVYLFDQESPTRELGEPFPGAKESPPTSLRGSPGSPSAPNRPQQADGSPNGSTAEEGGGFAWDDD FPLMTAKAAFAMALDPAAPAPAAPTPTPAPFSRFTVSPAPTSRFSITHVSDSDAESKRGPEAGAGGESKE A TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites1 | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_001080395 |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| RefSeq | NM_001080395.1, NP_001073864.1 |
| RefSeq Size | 5843 bp |
| RefSeq ORF | 4125 bp |
| Locus ID | 9625 |
| UniProt ID | Q6ZMQ8 |
| Cytogenetics | 17q25.3 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene contains a tyrosine kinase domain at the N-terminus and a proline-rich domain at the C-terminus. This gene is induced during apoptosis, and expression of this gene may be a necessary pre-requisite for the induction of growth arrest and/or apoptosis of myeloid precursor cells. This gene has been shown to produce neuronal differentiation in a neuroblastoma cell line. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011] |
| MW | 148.28 kDa |