Protein Modification for Phosphorylation Research

Protein Modification for Phosphorylation Research

INQUIRY

Creative BioMart offers comprehensive and high-quality protein modification services to accelerate kinase/phosphatase biology research and pharmaceutical development. Our services are tailored for each project to meet specific requirements.

Background

Protein modification refers to any change in the chemical composition of proteins. Co- and post-translational protein modifications are often responsible for the vast biodiversity in nature. These modifications play key roles in various important cellular processes, including differentiation, migration, signaling, and trafficking. Consequently, modifying proteins in a highly efficient and controlled manner would provide an invaluable tool for the study of their precise function and the interrogation and intervention of biological systems both in vitro and in vivo. The complementary use of both genetic and chemical methods offers great possibilities for the preparation of almost unlimited protein constructs with either natural or non-natural/modified residues.

Phosphorylation is one of the most common post-translational modifications. Dephosphorylation is the reverse reaction of phosphorylation. Kinases and phosphatases that responsible for the transfer of phosphate work in a balanced manner to regulate the function of proteins involved in a variety of important cellular functions. The diversity of kinases and phosphatases, multi-site phosphorylation, and the overlap of phosphorylation sites lead to diverse and complex patterns of protein phosphorylation. Kinase/phosphatase biology is extremely complex and challenging to study.

Figure 1. Proteins in eukaryotic cells can be edited after translation by a wide variety of reversible and irreversible PTM mechanisms. (Wang Y C, et al., 2014)

Available Services

Creative BioMart offers a full package of services to provide our customers with maximum flexibility. Proteins can be modified using various strategies, such as cleavage, crosslinking, denaturation, fragmentation, reduction of disulfides, and addition of new functional groups. Our scientific team is dedicated to helping our customers choose and optimize the most appropriate strategy for each project. Our services include, but are not limited to the following:

Co- and Post-Translational Modification
Acetylation		GPI anchoring		Phosphopantetheine
ADP-ribosylation		Hydroxylation		Phosphorylation
Amidation		Hypusine		Prenylation
Bromination		Iodination		Pyrrolidone carboxylic acid
Citrullination		Lysine topaquinone		Quinone
Cysteine tryptophylquinone		Methylation		S-nitrosylation
Dephosphorylation		Myristoylation		Sulfation
Formylation		Nitration		Topaquinone
Gamma-carboxyglutamic acid		Oxidation		Tryptophan tryptophylquinone
Glycosylation		Palmitoylation		Ubiquitylation/Sumoylation
Chemical Protein Modification
Modifications of natural amino acids		Modifications of unnatural amino acids		Site-selective modifications
Transition metal-free approaches		Transition metal-mediated approaches		Click chemistry approaches
Covalent modifications		Non-covalent modifications		PEGylation
Add-On Services
Protein Labeling			Protein Conjugation
Biotinylation	Desthiobiotinylation		APC conjugation		HRP conjugation
Fluorescent probe	Stable isotopes		Magnetic bead conjugation		Enzyme-protein conjugation
Active site probes	Protein tags		Protein-antibody conjugation		Small-molecule-protein conjugation
Gold nanoparticle labeling	Quantum dot labeling		Protein-metal chelator conjugation		Protein-synthetic polymer conjugation

Start from choosing one or more services above. If you can't find what you are looking for in the options above, please fill out the online inquiry form and let us know more details.

Custom Project Workflow

Why Choose Us

• The most comprehensive portfolio of services with maximum flexibility.
• Strong in-depth knowledge and extensive hands-on experience in protein modification.
• State-of-the-art techniques, scalable platform, and customized services for specific needs.
• Guaranteed quality, consistency, reliability, and reproductivity.
• Fast turnaround time and competitive price.

Creative BioMart has a strong team of chemists, biologists, and pharmacologists working on your project. We aim to provide our customers with the widest range of options to save their time and effort. The modified protein you need may already be available in our product catalog. If you require any further information, please feel free to contact us. We look forward to working together to meet your goals.

References

• Wang Y C, et al. Protein post-translational modifications and regulation of pluripotency in human stem cells. Cell research, 2014, 24(2): 143-160.
• Boutureira O & Bernardes G J L. Advances in chemical protein modification. Chemical reviews, 2015, 115(5): 2174-2195.