Chemical Synthesis of PhosphopeptidesINQUIRY
Creative BioMart offers overall solutions for the chemical synthesis of phosphopeptides, providing our customers with useful tools for the analysis of phosphorylation and cellular signaling events. We have a team of chemical biologists who specialize in applying chemical approaches to solve biological problems and can provide you with useful research tools and innovative ideas.
Introduction to Phosphopeptide Synthesis
Phosphorylation and dephosphorylation have been recognized as key events in cellular regulation and signal transduction. Since the low abundance of phosphorylated proteins poses difficulties for their separation, the efficient and site-specific synthesis of the relevant phosphopeptide that can mimic phosphorylated protein segments is a valuable alternative for investigating phosphorylation events in cellular processes. Phosphopeptides are very useful reagents for the study of phosphatase substrate specificity and protein-protein interactions. As a powerful complement to biological approaches, recent developments in chemical biology have made it possible to efficiently synthesize longer and multi-phosphorylated peptides, providing additional insights into phosphorylation-dependent mechanisms. It is foreseeable that chemists with the knowledge of phosphorylation chemistry will continue to make very important contributions to the understanding of the biological regulation of phosphorylation and the development of small molecule drugs.
Figure 1. General scheme for the phosphorylation of peptides by using the synthon approach (left) and using the building block approach (right). (Toth G K, et al., 2007)
Chemical Synthesis of Phosphopeptides Using Synthon Approach
Creative BioMart has developed an efficient protocol for the preparation of mono- and bis-protected phosphoamino acids used as building blocks for the solid-phase synthesis of phosphopeptides. With our extensive experience in the successful synthesis of phosphopeptides, we are well positioned to help our customers efficiently obtain high-quality phosphopeptides for downstream applications.
Chemical Synthesis of Phosphopeptides by Global Phosphorylation
Creative BioMart provides innovative solutions for chemical synthesis of phosphopeptides via global phosphorylation on the solid phase or in solution. Our chemists use their expertise to optimize reaction conditions and simplify synthetic routes to minimize or prevent side reactions and improve the efficiency of phosphopeptide synthesis.
Synthesis of Cyclic Phosphopeptides
Sterically hindered phosphorylated side-chain amino acid derivatives pose a significant difficulty in the synthesis of cyclic phosphopeptide. Creative BioMart has overcome this challenge by successfully developing a versatile, rapid, and simple approach to synthesize cyclic peptides used for probing signal transduction events.
Synthesis of multi-phosphorylated peptides
The synthesis of multi-phosphorylated peptides differs significantly from the synthesis of simple mono-, di- and tri-phosphopeptides. Creative BioMart offers several strategies for the preparation of multi-phosphorylated peptides to help our customers fully evaluate the impact of multi-phosphorylation on protein conformation and activity.
Custom Peptide Synthesis
Custom Peptide Conjugation and Modification
Peptide Analysis Services
Creative BioMart is a trusted solution provider with many years of experience in kinase/phosphatase biology research and drug development. We have always been committed to providing our customers with high-quality products and customized services to accelerate their cutting-edge research and drug development pipeline. We fully support your project every step of the way to success.
Contact us to see how our chemical biologists can help you with your project.
- Toth G K, et al. Phosphopeptides-Chemical Synthesis, Analysis, Outlook and Limitations. Current Organic Chemistry, 2007, 11(5): 409-426.
- McMurray J S, et al. The synthesis of phosphopeptides. Peptide Science: Original Research on Biomolecules, 2001, 60(1): 3-31.