Overall Solutions for Phosphoprotein AnalysisINQUIRY
As a trusted biotechnology solution provider, Creative BioMart offers comprehensive support for phosphoprotein analysis to help determine where, when, and how phosphorylation events occur. Our scientific team with extensive knowledge and experience in basic research and drug development can help our customers overcome obstacles and accelerate their success.
Introduction to Phosphoproteins
Phosphorylation is a key reversible post-translational modification that is involved in many essential cellular processes. The discovery and identification of phosphoproteins is critical to understanding the regulatory role played by phosphorylation. Phosphoproteins are usually generated by a reaction catalyzed by kinases. These proteins are part of the signal transduction cascade that amplifies and propagates cellular signals. Phosphoproteins are therefore important tools for elucidating the phosphorylation network and have been developed as biomarkers and potential therapeutics. Since the study of the dynamic phosphorylation process is challenging, comprehensive phosphoprotein and phosphorylation site analysis is necessary. In recent years, several novel experimental strategies have been developed to synthesize phosphoproteins and explore the phosphorylation status from individual proteins to phosphoproteomes. This has largely promoted the exploration of the biological role of phosphorylation and the understanding of the intricate cellular networks, which might reveal potential therapeutic strategies.
Figure 1. Schematic representation of phosphoprotein analysis workflow. (Delom F & Chevet E, 2006)
Phosphoprotein Separation and Detection
Creative BioMart offers a variety of techniques, such as two-dimensional gel electrophoresis, phosphate-affinity SDS-PAGE, and isotopic labeling, as well as validated protocols for the separation and detection of phosphoproteins and their nonphosphorylated counterparts both in vitro and in vivo.
Phosphoprotein Enrichment and Identification
Creative BioMart provides different enrichment strategies that can be performed at the phosphoprotein level or at the phosphopeptide level to help our customers overcome the challenges of identifying low-abundance phosphoproteins and keep their project moving forward.
Mapping and Analysis of Phosphorylation Sites
Creative BioMart has developed sensitive and quantitative assays for the mapping and analysis of phosphorylation sites and for the detection of phosphorylation events in vitro and in vivo. We have also established an advanced proteomics platform for large-scale phosphorylation analysis.
Creative BioMart provides comparative, quantitative, and large-scale phosphoproteome profiling strategies to analysis and validate the entire complement of phosphorylated proteins in cells that depend on the temporal and spatial balance of kinases and phosphatases.
Creative BioMart is a leading biotechnology company with many years of experience in kinase/phosphatase biology research and drug development. We provide a portfolio of high-quality products and customized services for the identification and characterization of phosphoproteins. We provide innovative researchers with a core technology platform covering a variety of advanced technologies such as IMAC, MS, MALDI, UPLC, and ESI, to help them turn their ideas into reality.
Contact us to see how we can help you with phosphoprotein analysis and drug development.
- Delom F & Chevet E. Phosphoprotein analysis: from proteins to proteomes. Proteome science, 2006, 4(1): 1-12.
- Duan H Z, et al. Unremitting progresses for phosphoprotein synthesis. Current Opinion in Chemical Biology, 2020, 58: 96-111.