Enzymatic Characterization of Phosphatases

Enzymatic Characterization of Phosphatases

INQUIRY

Creative BioMart offers a comprehensive selection of enzymatic characterization services to support in vitro phosphatase screening and profiling. Our talented scientific team provides our customers with unparalleled expertise and experience to help them overcome the challenges in research and drug development.

Background

Protein phosphorylation and dephosphorylation are the most prevalent modifications among the various types of post-translational modifications in prokaryotic and eukaryotic cells. They play important roles in regulating the structure and function and of proteins and enzymes in a wide range of cellular processes from cell fate control to metabolism regulation. The reaction catalyzed by phosphatases to remove the phosphate group from their substrates is functionally opposite to the reaction catalyzed by kinases. Phosphatases have been extensively studied and shown to be implicated in the pathogenesis of diseases such as cancer, diabetes, neurodegenerative diseases and more.

Studying phosphatases has been essential for elucidating phosphatase-substrate networks and thus providing more insights into the development of drugs. Enzymatic characterization is one critical part of phosphatase research. These measurements typically require the determination of pH and temperature optima, Michaelis constants, thermal stability, substrate specificity, requirements for cofactors, and the effect of ions on enzyme activity. Characterization of enzymatic properties of phosphatases can provide essential information for the identification of novel enzymes and the study of the mechanism of enzyme action.

Service Offering

Creative BioMart offers our customers a broad range of service options for enzymatic characterization of phosphatases to meet the specific requirements of both research and drug development. Our scientific team has extensive and successful experience in characterizing most members of the phosphatase family. If the following services do not meet your needs, please fill out the online inquiry form and we will develop customized assays tailored to your projects.

Determination of the Temperature and pH Optima

Temperature and pH are two important factors affecting enzyme activity. With our well-established experimental platform, our scientists can efficiently determine the temperature and pH optima for phosphatase activity. We can also provide visualization results of the overall relationship among temperature, pH, and enzyme activity.

Determination of Michaelis-Menten Enzyme Kinetics Parameters

Due to important functions of phosphatases, it becomes imperative to gain insight into the kinetics of their enzyme action. We help our customers measure the rate of catalytic reactions to establish kinetic parameters and enzyme activity of phosphatases.

Determination of Thermostability

Thermostability is an intrinsic property of enzymes, but may be influenced by external environmental factors such as cations, modulators, substrates, and co-enzymes. We provide a simple and effective way to determine thermostability of phosphatases.

Effects of Metal Ions on the Activity of Phosphatases

Metal ions play important roles in modulating the stability and activity of phosphatases. The effect of various metal ions, including but not limited to Mg²⁺, Ca²⁺, Mn²⁺, Fe³⁺, Co²⁺, Cu²⁺, Zn²⁺, Cd²⁺, on the activity of phosphatase can be evaluated according to customer requirements.

Creative BioMart is an advanced biotech company within the field of kinase/phosphatase biology. We offer our expertise in establishing assays for phosphatase screening & profiling. The extensive experience of our scientific team translates into the robust wealth of knowledge that we use to provide you with the highest quality of service. We provide efficient services with guaranteed turnaround times. If you have any questions or additional requirements, please do not hesitate to contact us.

References

• Cross M, et al. Enzyme characteristics of pathogen-specific trehalose-6-phosphate phosphatases. Scientific reports, 2017, 7(1): 1-9.
• Cheng H C, et al. Regulation and function of protein kinases and phosphatases. Enzyme research, 2011, 2011.