Mapping and Analysis of Phosphorylation SitesINQUIRY
Creative BioMart provides highly efficient services for mapping and analysis of phosphorylation sites in both eukaryotic and prokaryotic organisms to support the study of sophisticated phosphorylation events. We are equipped with advanced technology platforms and a scientific team with unparalleled expertise and experience in kinase/phosphatase biology and pharmaceutical research.
Kinases are responsible for phosphorylation events by transferring phosphate groups from ATP to specific substrates. Radiolabel studies have shown that many proteins in eukaryotic cells are modified by phosphorylation. Phosphorylation as the most common mechanism for regulating protein activity is involved in transmitting signals throughout the cell. Protein phosphorylation affects protein stability, protein cellular localization, and the ability of proteins to form stable complex with other molecules. Because of the important role phosphorylation plays in a wide range of cellular processes such as cell differentiation, cell cycle, and metabolism, abnormal phosphorylation events have been implicated in various diseases.
Elucidation of signaling networks is one of the most difficult challenges in biology and medicine. Mapping and analysis of phosphorylation sites on individual proteins and the proteome is an essential step to overcome these challenges. Generating a map that systematically describes all phosphorylation sites has been a major goal of current proteomic efforts. Several techniques have been developed to find out whether or not a protein is modified by phosphorylation. Detailed phosphorylation site analysis can provide clear information on functional relationships between signal proteins and the mechanism of phosphorylation. The method chosen for assessing phosphorylation may vary depending on factors such as the specific problem to be addressed and the availability of reagents and specialized equipment.
Figure 1. Tandem mass spectrometry (MS/MS) analysis of protein phosphorylation. (Dephoure N, et al., 2013)
Depending on the specific needs of our customers, Creative BioMart offers customized services based on different techniques to help them elucidate the mechanism of phosphorylation and develop novel drugs. Our experienced scientists will work closely with our customers to create the perfect plan for their project and accelerate their journey to success.
Prediction of Phosphorylation Sites
In silico methods for phosphorylation site prediction can provide useful and complementary information for phosphoproteome annotation. We provide a set of public and in-house bioinformatics tools for the prediction of kinase-specific substrates and their associated phosphorylation sites to facilitate both basic and translational research programs.
Precisely identification of which amino acid residues are phosphorylated is essential for understanding the underlying phosphorylation mechanisms. We have established proven and efficient phosphopeptide mapping strategies to recognizes sites of phosphorylation as well as to find changes in phosphorylation sites after reagent treatment.
Mass Spectrometry-based Methods
Mass spectrometry has emerged as a reliable and sensitive method for phosphorylation site mapping. Equipped with state-of-the-art instrument and equipment (MALDI-TOF, LC/MS, ESI/MS, etc), our mass spectrometry platform enables targeted phosphorylation site identification and quantification. We can also combine mass spectrometry with tradition approaches such as radioactive labeling to achieve more comprehensive phosphorylation site analysis.
Interpretation of Phosphorylation Site Mapping Data
Methods for mapping phosphorylation sites, especially mass spectrometry, generate a large amount of raw data. Our scientists use their expertise and experience to provide our customers with high-quality data interpretation services and help them achieve their required outcomes.
- Mapping sites on isolated proteins.
- Large-scale proteomic analysis of phosphorylation.
- Rigorous, high-confidence mapping results.
- Analysis of the functions and phenotypes of phosphorylation-site mutants.
Creative BioMart is an advanced biotech company within the field of kinase/phosphatase biology. We offer our expertise in establishing assays for phosphorylation analysis. The extensive experience of our scientific team translates into the robust wealth of knowledge that we use to provide you with the highest quality of service. We can also help our customers develop custom phosphorylation-specific antibodies. If you are interested in our services or have any additional questions, please do not hesitate to contact us.
- Dephoure N, et al. Mapping and analysis of phosphorylation sites: a quick guide for cell biologists. Molecular biology of the cell, 2013, 24(5): 535-542.
- Knight Z A, et al. Phosphospecific proteolysis for mapping sites of protein phosphorylation. Nature biotechnology, 2003, 21(9): 1047-1054.