Phosphatase Activity Assays

Phosphatase Activity Assays

Phosphatase Activity Assays


Creative BioMart offers a comprehensive selection of phosphatase activity assays to support in vitro phosphatase screening and profiling. Our talented scientific team provides our customers with unparalleled expertise and experience to help them overcome the challenges in research and drug development.


Protein phosphorylation is crucial component of cellular signal transduction. In the vast majority of cases, it is a reversible post-translational modification (PTM). Changes in the phosphatase state of a protein can be the result of a change in the activity of either the relevant kinase(s) or phosphatase(s). Phosphatases play highly regulated and very active roles in many cellular processes. They are very important in setting the levels of protein phosphorylation. Phosphatases have been shown to play a key role in health and disease and are therefore potential drug targets. The study of phosphatases is an important but challenging task.

Phosphatase biology has thrived in the last few years. To date, several activity assays have been developed based on the biological function of phosphatases. Conventional methods have provided crucial insights into the substrates and mechanisms of phosphatases as well as their exclusive functions in highly complex networks. Today, advances in cellular imaging, chemical biology, and other technological approaches have provided new insights into cell signaling studies and the development of therapeutics. However, the continuous development of assay formats has led to higher demand for precision and sensitivity. New idealized detection strategies also need continue to be developed to meet practical demands.

Illustration of two classic methods for ALP activities assay.

Figure 1. Illustration of two classic methods for ALP activities assay. (Tang Z, et al., 2019)

Assay Formats

Creative BioMart offers our customers a wide range of options for phosphatase activity assays to help them accomplish the objective of their research and accelerate the overall drug development process. If you can’t find an assay of your interest, please fill out the online inquiry form and let us know more details.

Assay Formats


Colorimetric Assays

  • Simple operation, quick response, and adaptable sensitivity.
  • Free of complicated or expensive instruments.
  • Directly visual outcome for qualitative or semiqualitative identification.

Fluorescence-Based Assays

  • Unmatched sensitivity, stability, and flexibility.
  • Lower requirements of sample amounts.
  • Diverse selection of fluorescent substances such as organic dyes, nanomaterials, and fluorescent DNA probes.

Bioluminescent Assays

  • Homogeneous and stable.
  • High sensitivity, broad linearity, and robustness for high-throughput applications.

Radiometric Assays

  • High specificity and sensitivity.
  • Applicable to a wider range of substrate concentrations.

Label-Free Assays

  • Low production cost.
  • Fast response and high sensitivity.
  • Compatible with real-time detection.

Service Offering

Creative BioMart offers a broad range of activity assay formats for almost all members of the phosphatase family. Our scientific team will work closely with our customers to develop an optimal strategic plan.

Service Offering of Phosphatase Activity Assays – Creative BioMart

Creative BioMart is an advanced biotech company within the field of kinase/phosphatase biology. We offer our expertise in establishing assays for phosphatase screening & profiling. The extensive experience of our scientific team translates into the robust wealth of knowledge that we use to provide you with the highest quality of service. We provide efficient services with guaranteed turnaround times. If you have any questions or additional requirements, please do not hesitate to contact us.


  • Tang Z, et al. Assays for alkaline phosphatase activity: progress and prospects. TrAC Trends in Analytical Chemistry, 2019, 113: 32-43.
  • Mustelin T. A brief introduction to the protein phosphatase families. Protein phosphatase protocols, 2007: 9-22.
For research use only. Not intended for any clinical use.