Phosphorylation-dependent Protein Subcellular Localization

Phosphorylation-dependent Protein Subcellular Localization

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Phosphorylation-dependent Protein Subcellular Localization


Creative BioMart provides professional protein subcellular localization services to accelerate kinase/phosphatase biology research and pharmaceutical development. Our in-depth knowledge, state-of-the-art techniques, and extensive hands-on experience allow us to deliver high-quality and reliable results within a guaranteed time.


All cells are comprised of well-defined compartments with specific functions. The efficiency of many subcellular processes is increased by the fact that compartmentalization concentrates the required components to a confined space within the cell. Proteins are responsible for virtually every cellular process. Compartmentalization provides a specific environment for proteins to perform specific functions. Confinement to a specific compartment can also increase specificity. The most common subcellular localizations include the cytoplasmic membrane, the cell wall, the cytoplasma, and the extracellular environment. Abnormal localization of proteins can easily lead to disruption of cellular activities. Therefore, understanding the subcellular localization of proteins is necessary to study the functions of genes, protein interactions, and their mechanisms of action.

Phosphorylation is one of the main types of covalent modifications and is also recognized as the most frequent modification. Phosphorylation and dephosphorylation are important processes necessary for the regulation of subcellular localization. Subcellular localization is also involved in determining the signal output of signaling pathways mediated by kinases and phosphatases. Revealing the subcellular localization of kinases, phosphatases and other proteins involved in phosphorylation and dephosphorylation is a crucial step in kinase/phosphatase biology research and the development of therapies or biomarkers for phosphorylation-related disease

Subcellular localization of VIP1 variants.Figure 1. Subcellular localization of VIP1 variants. (Takeo K & Ito T, 2017)


  • Understanding protein function and dissecting intracellular trafficking.
  • Identifying abnormal subcellular localization of disease-associated proteins
  • Revealing mechanisms of disease progression.
  • Significantly improving target identification in the drug discovery process.
  • Developing potential diagnostic targets and vaccine candidates.

Available Services

Creative BioMart offers different strategies and scalable platforms for protein subcellular localization in different species, including mammalian, plant, insect, bacteria, fungi, yeast and algae, to meet the specific requirements of each project. Our scientific team has the ability to ensure reproducible results. Our services include, but are not limited to the following:

Featured Services Featured Platforms
Subcellular localization prediction Online resources, software, and databases
In situ hybridization Electron microscopy
Immunofluorescence Fluorescence microscopy
Fluorescent imaging Live-cell fluorescence microscopy
Fluorescence resonance energy transfer (FRET) imaging Confocal laser scanning microscopy
Isotope tagging (LOPIT) Mass spectrometry
Immunogold labeling Immunogold electron microscopy
Subcellular colocalization analysis High-throughput image analysis
Add-On Services Types of Samples
Protein tagging Cell cultures
Fusion protein expression Tissue samples
Live-cell protein labeling Living cells
Protein analysis In vivo samples

If you can't find what you are looking for in the options above, please fill out the online inquiry form and let us know more details.

Custom Project Workflow

Custom Project Workflow – CD BioSicences

Creative BioMart has a strong team of chemists, biologists, and pharmacologists working on your project to save your time and effort. If you require any further information, please feel free to contact us. We look forward to working together to meet your goals.


  • Takeo K & Ito T. Subcellular localization of VIP 1 is regulated by phosphorylation and 14-3-3 proteins. FEBS letters, 2017, 591(13): 1972-1981.
  • Simeunovic A, et al. Know where your clients are: subcellular localization and targets of calcium-dependent protein kinases. Journal of experimental botany, 2016, 67(13): 3855-3872.
For research use only. Not intended for any clinical use.