Custom Protein Production for Phosphorylation ResearchINQUIRY
Creative BioMart offers numerous options for custom protein production services to accelerate kinase/phosphatase biology research and pharmaceutical development. Our extensive experience in protein production allows us to deliver high-quality proteins and provide comprehensive services within a guaranteed time.
As an essential part of living organisms, proteins play an important role in virtually every process within cells, such as cell signaling, cell cycle, cell adhesion, cell metabolism, and immune responses. The study of protein structure and function has become the focus of current research. Many proteins have become very attractive sources for therapeutic development due to their high intrinsic potency and selectivity for biological targets. The production of specific proteins is usually achieved by manipulating gene expression in an organism. It has been developed as a widely used biotechnological process in life sciences, biotechnology and medicine.
Protein phosphorylation is an extremely important regulatory mechanism in various cellular processes. Kinases and phosphatases are drivers of protein phosphorylation and dephosphorylation events. Production of kinases and phosphatases as well as their substrates is essential to study the mechanism of protein phosphorylation and the development of drugs targeting kinase/phosphatase signaling networks.
Figure 1. Schematic representation of hTLK1B-pETDUET-1-Lambda Protein Phosphatase expression vector (A) and coexpression of recombinant 6×His-tagged hTLK1B along with bacteriophage lambda protein phosphatase at 25 °C at different IPTG concentrations. (Bhoir S, et al., 2018)
Available Expression Systems
Both cell-based and cell-free systems are available at Creative BioMart. We provide our customers with maximum flexibility to meet their needs in the life sciences and pharmaceutical industry.
|Cell-Based Expression Systems||Cell-Free Expression Systems|
|Bacteria expression system||Escherichia coli extract (ECE)|
|Yeast expression system||Rabbit reticulocyte lysate (RRL)|
|Insect expression system||Saccharomyces cerevisiae extract (SCE)|
|Algal expression system||Insect cell extract (ICE)|
|Plant-based expression system||Wheat germ extract (WGE)|
|Mammalian expression system||Tobacco BY-2 cell extract (BYE)|
|Transient protein expression||CHO, K562, and heLa extracts|
|Stable protein expression||Leishmania tarentolae extract (LTE)|
|Cell-based membrane protein expression||Cell-free membrane protein expression|
|High-cell-density protein expression system||Chemical protein synthesis|
Creative BioMart offers a full package of services for custom protein production to meet any research need. Our scientific team is dedicated to helping our customers choose and optimize the most appropriate approach to generate proteins with high purity and high yields for each project. Our services include, but are not limited to the following:
|Phase Ⅰ Services||Phase Ⅱ Services||Add-On Services|
|Gene synthesis and cloning||High yield protein production||Protein labeling and conjugation||Tag removal|
|Cell line development||Fermentation||Protein refolding||Protein engineering|
|Protein expression||Endotoxin removal & testing||Post-translational modification||Functional assays|
|Protein purification||QC & delivery||Assay development||Antibody development|
Start from choosing one or more services above. If you can't find what you are looking for in the options above, please fill out the online inquiry form and let us know more details.
- Protein production is under strict quality control. QC reports will be delivered along with your proteins.
- Proteins will be delivered in the format you need.
- For long-term storage, proteins should be stored at -20°C or colder and away from bright light after receiving.
Custom Project Workflow
Why Choose Us
- Extensive experience working with various recombinant expression systems and protein classes.
- Up-to-date knowledge in protein technologies and state-of-the-art techniques.
- Guaranteed quality, consistency, reliability, and reproductivity.
- Scalable platform and customized services for specific needs.
- Fast turnaround time and competitive price.
Creative BioMart has a strong team of chemists, biologists, and pharmacologists working on your project to save your time and effort. Your protein may already be available in our product catalog. If you require any further information, please feel free to contact us. We look forward to working together to meet your goals.
- Bhoir S, et al. High yield bacterial expression, purification and characterisation of bioactive Human Tousled-like Kinase 1B involved in cancer. Scientific reports, 2018, 8(1): 1-9.
- Hunt I. From gene to protein: a review of new and enabling technologies for multi-parallel protein expression. Protein expression and purification, 2005, 40(1): 1-22.