Antibody Validation for Phosphorylation Research

Antibody Validation for Phosphorylation Research

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Antibody Validation for Phosphorylation Research


Creative BioMart is committed to accelerating kinase/phosphatase biology research and pharmaceutical development projects by expanding our antibody validation methods to ensure antibody quality and reliability. As a leading biotechnology company with extensive experience in antibody development, we provide our customers with tailor-made services specifically for each specific need.

Introduction to Antibody Validation

Antibodies are one of the most critical research and diagnostic reagents in life sciences and medicine. A "good" antibody is one that binds specifically to the right target in a particular application. Regardless of the field and application, however, a great deal of time, effort, and expense is still expended to monitor false positive or false negative results in antibody-based experiments, or to ensure that the antibodies from commercial sources do live up to expectations. Therefore, it is necessary to thoroughly validate antibodies and disclose the results transparently to enhance the reliability and utility of studies using antibodies.

The reproducibility of studies using antibodies depends on their correct validation. Ensuring correct antibody validation is an urgent concern for researchers, clinical users and commercial producers. Antibodies should be validated in terms of target specificity, suitability for specific applications, binding affinity, and reproducibility. Moreover, validation needs to be updated for different forms and batches of antibody products. Standardized and agreed procedures are required to ensure antibody quality and address the reproducibility crisis.

Development and validation of a polyclonal antibody against PY-PTH1R.

Figure 1. Development and validation of a polyclonal antibody against PY-PTH1R. (Qiu T, et al, 2018)

Service Options

Creative BioMart fully recognizes the importance of highly validated antibodies and data reproducibility. We have been working extensively on antibody validation and offer our customers with trusted services. Simply tell us your targets of interest and expected applications, we will validate the antibody you provide or do the antibody hunting for you.

Validation of Antibody Specificity

We provide antibody validation services covering both target specificity and the suitability of an antibody for a specific application.

Target Specificity Application-Specific Validation
Knockout cell lines and animal models Western blot (WB)
Knockdown cell lines and animal models ELISA
Cell treatment Immunohistochemistry (IHC)
Immunoprecipitation-mass spectrometry (IP-MS) Immunocytochemistry (ICC)
Protein or peptide array Immunofluorescence (IF)
Protein expression profile Immunoprecipitation (IP)
Targeted proteomics Chromatin immunoprecipitation (ChIP)
Neutralization Flow cytometry
Independent antibody strategies Antibody arrays

Determination of Antibody Affinity

Antibody affinity refers to the strength of the interaction between an antibody and a specific epitope and is typically measured by the equilibrium dissociation constant (KD). It is another important parameter for antibody validation. Creative BioMart provides several techniques to determine antibody affinity, including but not limited to ELISA, surface plasmon resonance (SPR), bio-Layer interferometry (BLI), and microscale thermophoresis (MST).

Antibody Reproducibility

It is critical for researchers to ensure reproducibility of antibodies to consistently get reproducible data, especially when using polyclonal antibodies, as even different batches of antibodies from the same company may perform differently. Creative BioMart can help customers test new batches of antibodies in parallel with existing batches or compare antibodies from different commercial sources.

* If you have additional questions about our services, please fill out the online inquiry form and let us know more details.

Creative BioMart' scientific team has accumulated extensive experience in handling numerous antibody validation projects. We can help our customers choose the most appropriate and effective strategy to match their specific needs. We provide not only antibody validation services, but also a full range of antibody production services starting from antigen preparation. A large collection of validated antibodies is also available. If you are interested in our services, please do not hesitate to contact us.


  • Qiu T, et al. IGF-I induced phosphorylation of PTH receptor enhances osteoblast to osteocyte transition. Bone research, 2018, 6(1): 1-12.
  • Taussig M J, et al. Antibody validation: a view from the mountains.New biotechnology, 2018, 45: 1-8.
For research use only. Not intended for any clinical use.