Kinase Functional AssaysINQUIRY
Creative BioMart offers a wide range of kinase functional assays to help our customers advance their kinase research and drug discovery. With extensive knowledge and experience in kinase screening & profiling, we can help our customers resolve issues and overcome challenges.
Protein phosphorylation is an important cellular regulatory mechanism. A broad range of receptors and enzymes can be activated/deactivated by phosphorylation/dephosphorylation events. Kinases are responsible for protein phosphorylation. They catalyze the transfer of phosphate from a donor ATP molecule to a wide variety of cellular substrates, resulting in the formation of phosphorylated products and ADP. Kinases themselves can also be regulated by other kinases and initiate cascade reactions. Because of their important role in many cellular processes, kinases have been one of the most widely studied enzyme families in biological and medical research. Hyperactive or malfunctioning kinases have also been associated with various diseases such as cancer, diabetes, and cardiovascular diseases, making them important targets for drug discovery.
The rapidly growing interest in kinases research and drug discovery has prompted the development of many different assay technologies to monitor changes in kinase activity. Kinase functional assays focus on detecting kinase activity by directly or indirectly quantifying either the formation of the phosphorylated product or the amount of ATP used or ADP formed. Compared to binding assays and cellular assays, kinase functional assays are the most preferred assay format. The commonly used functional assays include colorimetric assays, bioluminescent assays, fluorescence-based assays, radiometric assays, label-free assays, and mobility shift assays. Understanding different format of kinase functional assays is the first and key step in elucidating kinase signaling and developing successful drugs.
Figure 1. Phospho-signaling networks. (Ardito F, et al., 2017)
Creative BioMart offers our customers a wide range of options for kinase functional assays to help them accomplish the objective of their research. Our scientific team will work closely with our customers to develop an optimal strategic plan. If you can't find an assay of your interest, please fill out the online inquiry form and let us know more details. We can offer you our expertise to help you accelerate your success.
Colorimetric Assays for Kinase Activity
Colorimetric assays have gained great interest due to their inherent advantages, including low cost, simple operation, adaptable sensitivity, quick response, wide linear range, and the possibility of qualitative or semiqualitative identification by the naked eye.
Bioluminescent Assays for Kinase Activity
Luminescence-based kinase assays can be used to measure the depletion of ATP. Bioluminescent assays are homogeneous and stable, offering the advantages of sensitivity, broad linearity, and robustness for high-throughput screening (HTS) applications.
Fluorescence-Based Assays for Kinase Activity
Fluorescence offers unmatched sensitivity, stability, and flexibility compared to many other techniques. The diversity of available fluorescent dyes provides virtually unlimited possibilities for assay design. These advantages make fluorescence-based assays one of the easiest and popular methods for kinase activity studies.
Radiometric Assays for Kinase Activity
Radiometric assays allow direct measurement of the rate of a given kinase reaction to accurately determine and quantify its catalytic activity. This assay format is versatile and compatible with virtually any type of kinase substrates, as long as there is a suitable method to separate radiolabeled ATP and substrate.
Label-Free Assays for Kinase Activity
Label-free methods have been developed and attracted increasing attention in the development of kinase assays due to their low production cost, pathway-unbiased, fast response, and high sensitivity. These assays provide a new dimension to elucidate kinase biology and facilitate drug discovery.
Mobility Shift Assays
The ability of mobility shift assays to real-time monitoring the activity and kinetics of kinases, coupled with the fact that this technique allows for accurate quantitation of both substrates and products, makes it a good candidate for kinetic and mechanistic studies of kinases.
Creative BioMart is an advanced biotech company within the field of kinase/phosphatase biology. We offer our expertise in establishing assays for kinase screening & profiling. We provide efficient services with guaranteed turnaround times. Any data or details you provide will be kept strictly confidential. If you have any questions or additional requirements, please do not hesitate to contact us and let us know how we can help.
- Ardito F, et al. The crucial role of protein phosphorylation in cell signaling and its use as targeted therapy. International journal of molecular medicine, 2017, 40(2): 271-280.
- Jia Y, et al. Current in vitro kinase assay technologies: the quest for a universal format. Current drug discovery technologies, 2008, 5(1): 59-69.