Ligand Binding AssaysINQUIRY
Creative BioMart offers multiple service options for ligand binding assays to help our customers advance their kinase research and drug discovery. With extensive knowledge and experience in kinase screening & profiling, we can help our customers resolve issues and overcome challenges.
Kinases play a key role in the regulation of signal transduction and many other cellular processes. Most of these physiological processes are regulated by molecular mechanisms involving kinase-ligand interactions. Ligand molecules can induce conformational changes to regulate kinase activity. Since kinases have been intensively studied as potential therapeutic targets for various diseases, quantification of ligand binding to kinases by ligand binding assays is essential not only for understanding biological functions of kinases but also for developing new drugs based in large part on binding affinity and efficacy. Every step of kinase-ligand interactions can be analyzed by different ligand-binding assays. The data of in vitro ligand binding may also provide important information about in vivo efficacy of these ligands.
Figure 1. Upon ligand binding, receptor tyrosine kinases (RTKs) dimerize and become activated. (Zinkle A & Mohammadi M, 2018)
Creative BioMart has a scientific team dedicated to the study of binding properties, including to binding affinity, binding sites, ligand efficiency, conformations of targets and binding kinetics and thermodynamics. We offer a comprehensive techniques and advanced equipment for ligand binding assays. Our scientific team will help our customers develop an optimal strategic plan to meet the specific requirements of their projects.
Labeled ligand binding assays are valuable in determining the binding affinity, kinetics and thermodynamics, ligand efficiency, and molecular conformations of targets. The development, implementation, and validation of reliable labeled ligand binding assays to characterize kinase binding properties is essential for successful drug discovery. Creative BioMart provides our customers with fluorescent, radiometric, and bioluminescent ligand binding assays to choose from for different research purposes.
Label-free detection technologies that do not require labeling of target molecules have been widely used to characterize biomolecular interactions and can be applied to drug candidate screening when combined with high-throughput capability. Label-free ligand binding assays provide a new dimension for elucidating kinase biology and facilitating drug discovery. Creative BioMart offers advanced techniques for label-free ligand binding assays, including SPR, RWG, BLI, and mass spectrometry.
Structure-based ligand binding assays provide a unique window into the role of conformational dynamics in kinase regulation and function, and are therefore of great importance for drug design. Structural information has provided insights into the development of inhibitors targeting the inactive or active form of the kinase. Creative BioMart offers our expertise in the identification of conformational changes and binding sites using X-ray crystallography, NMR, and cryo-EM.
Creative BioMart is an advanced biotech company within the field of kinase/phosphatase biology. We offer our expertise in establishing assays for kinase screening & profiling. We provide efficient services with guaranteed turnaround times. Any data or details you provide will be kept strictly confidential. If you have any questions or additional requirements, please fill out the online inquiry form and let us know how we can help.
- Zinkle A & Mohammadi M. A threshold model for receptor tyrosine kinase signaling specificity and cell fate determination. F1000Research, 2018, 7.
- Wang Y & Ma H. Protein kinase profiling assays: a technology review. Drug Discovery Today: Technologies, 2015, 18: 1-8.