Protein Analysis Services for Phosphorylation Research
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INQUIRYCreative BioMart offers numerous options for protein analysis services to accelerate kinase/phosphatase biology research and pharmaceutical development. Our extensive experience in protein analysis allows us to provide comprehensive, high-quality services and reliable results within a guaranteed time.
Background
Proteins, also known as polypeptides, are macromolecules made from long chains of amino acid residues. The differences in the type, number, and sequence of amino acid residues and the three-dimensional structure of proteins determine their different physicochemical properties and biological activities. Proteins are the major players in virtually all cell functions. The main characteristic of proteins is their ability to specifically bind tightly to other molecules, giving them a diverse range of functions. A broad range of experimental techniques for the detection, purification, identification, and characterization can be used to perform protein analysis, providing useful information about the purity, structure, conformation, properties, and activities of proteins.
Protein phosphorylation is tightly associated with protein activity and is of fundamental importance in biological regulation. Different kinases and phosphatases are involved in protein phosphorylation, allowing for combinatorial regulation at the post-translational level. Therefore, in-depth analysis of kinases, phosphatases, and their protein substrates is indispensable for understanding the regulatory mechanisms of multiple biological processes.
Figure 1. Workflow of expression, affinity purification, and top-down LC-MS analysis of the recombinant PKA C-subunit. (Wu Z, et al., 2019)
Available Services
Creative BioMart offers a full package of protein analysis services to provide our customers with maximum flexibility. Our scientific team is dedicated to helping our customers choose and optimize the most appropriate approach for each project. Our services include, but are not limited to the following:
| Chemical & Physical Analysis | |
| Protein purity analysis | Protein molecular weight determination |
| Detection of protein charge isoforms | Protein sequence analysis |
| Prediction and analysis of protein solubility | Protein stability testing |
| Protein quantification | Protein structure prediction and determination |
| Protein identification | Protein aggregation analysis |
| Residual solvent analysis | Measurement of residual moisture content |
| Biological Analysis | |
| Sterility testing | Endotoxin control & analysis |
| Biosafety testing | Bioburden determination |
| Protein function prediction | Structure activity relationship (SAR) analysis |
| Protein mapping | Protein post-translational modification analysis |
| Protein interaction analysis | Screening and profiling |
| Safety evaluation | In vivo analysis |
| In Silico Analysis | |
| Bioinformatics | Cheminformatics |
| Proteomics | Phosphoproteomics |
| Featured Platforms | |
| Mass spectrometry | MALDI-TOF/MS |
| LC-MS and LC-MS/MS | HPLC and UHPLC |
| Nuclear magnetic resonance (NMR) | X-ray crystallography (XRC) |
| Cryo-electron microscopy (cryo-EM) | Circular dichroism (CD) spectroscopy |
| Analytical ultracentrifuge (AUC) | Differential Scanning Calorimetry (DSC) |
| Surface plasmon resonance (SPR) | Dynamic light scattering (DLS) |
| Affinity chromatography | Fourier transform infrared spectroscopy (FTIR) |
| Hydrophilic interaction chromatography (HILIC). | Reversed phase chromatography (RPC) |
| Multi-detection GPC/SEC | In silico tools and databases |
Start from choosing one or more services above. If you can't find what you are looking for in the options above, please fill out the online inquiry form and let us know more details.
Custom Project Workflow
Why Choose Us
- The most comprehensive portfolio of services with maximum flexibility.
- Strong in-depth knowledge, state-of-the-art techniques, and extensive hands-on experience.
- Scalable platform and customized services for specific needs.
- Guaranteed quality, reliability, and reproductivity.
- Fast turnaround time and competitive price.
Creative BioMart has a strong team of chemists, biologists, and pharmacologists working on your project. We aim to provide our customers with the widest range of options to save their time and effort. If you require any further information, please feel free to contact us. We look forward to working together to meet your goals.
References
- Wu Z, et al. Comprehensive characterization of the recombinant catalytic subunit of cAMP-dependent protein kinase by top-down mass spectrometry. Journal of the American Society for Mass Spectrometry, 2019, 30(12): 2561-2570.
- Peck S C. Analysis of protein phosphorylation: methods and strategies for studying kinases and substrates. The Plant Journal, 2006, 45(4): 512-522.