Analysis of Phosphorylation In Vitro

Analysis of Phosphorylation In Vitro

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Analysis of Phosphorylation In Vitro


Creative BioMart provides expertise and experience in the comprehensive analysis of phosphorylation in vitro to support basic biological research and drug development. We offer state-of-the-art techniques and equipment to help our customers overcome the challenges and bottlenecks of elucidating phosphorylation events.


Kinases and phosphatases are responsible for phosphorylation and dephosphorylation, respectively. Phosphorylation is of particular importance in biology because it is a key reaction in protein and enzyme function, glycolysis, and energy storage and release. Many types of molecules can be phosphorylated. Protein phosphorylation, glucose phosphorylation, and oxidative phosphorylation are the most important types of phosphorylation. Phosphorylation plays a key regulatory role in both prokaryotic and eukaryotic cells. It has long been recognized as an essential mechanism for controlling cellular function. In particular, protein kinases are prominent in cellular signaling transduction. Their hyperactivity, malfunction or overexpression has been implicated in several diseases, such as cancer. Therefore, analysis of phosphorylation is important to elucidate its mechanisms. A wide range of methods and techniques such as electrophoresis, isotope labeling, immunoassays, and mass spectrometry have been developed to determine whether a molecule is phosphorylated and to further identify and characterize the phosphorylation sites.

Phospho-signaling networks.

Figure 1. Phospho-signaling networks. (Ardito F, et al., 2017)

Technical Capabilities

With extensive knowledge and unparalleled experience in kinase/phosphatase biology, we have established an integrated technology platform with the largest portfolio of assay formats, enabling our scientific team to offer customized services to meet your individual needs.

Technical Capabilities of Analysis of Phosphorylation In Vitro – Creative BioMart

Our Services

Creative BioMart offers comprehensive services for phosphorylation analysis to support our customers’ cutting-edge research and accelerate their journey to success. If you have any additional requirements, please fill out the online inquiry form and let us know more details.

Creative BioMart has extensive knowledge and years of experience in kinase/phosphatase biology and pharmaceutical development. Equipped with state-of-the-art facilities and technology, we are able to deliver trustworthy and comprehensive phosphorylation analysis services to meet our customers’ requirements. We guarantee that any data or details you provide will be kept strictly confidential. If you are interested in our services, please do not hesitate to contact us.


  • Ardito F, et al. The crucial role of protein phosphorylation in cell signaling and its use as targeted therapy. International journal of molecular medicine, 2017, 40(2): 271-280.
  • Olive D M. Quantitative methods for the analysis of protein phosphorylation in drug development. Expert review of proteomics, 2004, 1(3): 327-341.
For research use only. Not intended for any clinical use.