Substrate Profiling of Phosphatases

Substrate Profiling of Phosphatases

Substrate Profiling of Phosphatases


Creative BioMart offers customized substrate profiling services to support in vitro phosphatase screening and profiling. Our talented scientific team provides our customers with unparalleled expertise and experience to help them overcome the challenges in research and drug development.


Kinases and phosphatases responsible for phosphorylation and dephosphorylation, respectively, are linked by their common substrates to form sophisticated signaling networks that play crucial roles in regulating cellular processes. Phosphatases participate in cellular processes by dephosphorylating their substrates. The catalytic domains of kinases are structurally conserved, whereas those of phosphatases are more diverse with different catalytic mechanisms, and thus phosphatases are less understood. Typically, phosphatases can be classified into protein Ser/Thr phosphatases and protein tyrosine phosphatase based on their general substate specificity.

Members of the phosphatase family have been shown to play key roles in fundamental biological processes and be involved in the pathogenesis of various diseases. Although much progress has been made in elucidating phosphatase-substrate networks, the identification of substrates for these important enzymes is still a challenging task. The physiological substrates of many phosphatases are still unknown. Substrate profiling in the context of their complex biological networks can provide essential information to abstract phosphatase-substrate specificities and reveal potential substrate recognition criteria.

Methods for phosphatase substrate identification.

Figure 1. Methods for phosphatase substrate identification. (Fahs S, et al., 2016)

Service Offering

Creative BioMart provides our customers with a robust, reliable, and high-quality one-stop services for efficient profiling of phosphatase substrates to accelerate their research and drug development efforts. Our services include, but are not limited to the following:

  • Phosphatase Substrate Identification

    Phosphatase substrate identification remains challenging because of their complex substrate profiles, the presence of regulatory elements for substrate recognition, and the highly transient phosphatase-substrate interactions.
    Creative BioMart provides both mass spectrometry-based methods and intact cell-based methods to identify novel substrates for phosphatases and define phosphatase-substrate interactions, providing valuable insights into cell signaling.

  • Phosphatase Substrate Specificity Profiling

    Substrate specificity is an important property of enzymes. The rules by which phosphatases are able to recognize such a wide range of substrates have not been fully elucidated.
    Creative BioMart provides different strategies such as substrate phage libraries, peptide libraries, microarrays, and multiplex substrate profiling to help our customers define phosphatase substrate specificity in more detail and elucidate phosphatase-substrate networks.

Creative BioMart is an advanced biotech company within the field of kinase/phosphatase biology. We offer our expertise in establishing assays for phosphatase screening & profiling. The extensive experience of our scientific team translates into the robust wealth of knowledge that we use to provide you with the highest quality of service. We provide efficient services with guaranteed turnaround times. If you have any questions or additional requirements, please do not hesitate to contact us.


  • Fahs S, et al. Approaches to study phosphatases. ACS chemical biology, 2016, 11(11): 2944-2961.
  • Tiganis T & Bennett A M. Protein tyrosine phosphatase function: the substrate perspective. Biochemical Journal, 2007, 402(1): 1-15.
For research use only. Not intended for any clinical use.