CAMKK2 (NM_153499) Human Tagged ORF Clone
Product Information
| Product Name | CAMKK2 (NM_153499) Human Tagged ORF Clone |
| Cat.No | CDEP1006 |
| Description | CAMKK2 (Myc-DDK-tagged)-Human calcium/calmodulin-dependent protein kinase kinase 2, beta (CAMKK2), transcript variant 2 |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | CAMKK2 |
| Synonyms | CAMKK; CAMKKB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Sequence Data | ORF Nucleotide Sequence (showhide) >RC219152 representing NM_153499 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCATCATGTGTCTCTAGCCAGCCCAGCAGCAACCGGGCCGCCCCCCAGGATGAGCTGGGGGGCAGGG GCAGCAGCAGCAGCGAAAGCCAGAAGCCCTGTGAGGCCCTGCGGGGCCTCTCATCCTTGAGCATCCACCT GGGCATGGAGTCCTTCATTGTGGTCACCGAGTGTGAGCCGGGCTGTGCTGTGGACCTCGGCTTGGCGCGG GACCGGCCCCTGGAGGCCGATGGCCAAGAGGTCCCCCTTGACTCCTCCGGGTCCCAGGCCCGGCCCCACC TCTCCGGTCGCAAGCTGTCTCTGCAAGAGCGGTCCCAGGGTGGGCTGGCAGCCGGTGGCAGCCTGGACAT GAACGGACGCTGCATCTGCCCGTCCCTGCCCTACTCACCCGTCAGCTCCCCGCAGTCCTCGCCTCGGCTG CCCCGGCGGCCGACAGTGGAGTCTCACCACGTCTCCATCACGGGTATGCAGGACTGTGTGCAGCTGAATC AGTATACCCTGAAGGATGAAATTGGAAAGGGCTCCTATGGTGTCGTCAAGTTGGCCTACAATGAAAATGA CAATACCTACTATGCAATGAAGGTGCTGTCCAAAAAGAAGCTGATCCGGCAGGCCGGCTTTCCACGTCGC CCTCCACCCCGAGGCACCCGGCCAGCTCCTGGAGGCTGCATCCAGCCCAGGGGCCCCATTGAGCAGGTGT ACCAGGAAATTGCCATCCTCAAGAAGCTGGACCACCCCAATGTGGTGAAGCTGGTGGAGGTCCTGGATGA CCCCAATGAGGACCATCTGTACATGGTGTTCGAACTGGTCAACCAAGGGCCCGTGATGGAAGTGCCCACC CTCAAACCACTCTCTGAAGACCAGGCCCGTTTCTACTTCCAGGATCTGATCAAAGGCATCGAGTACTTAC ACTACCAGAAGATCATCCACCGTGACATCAAACCTTCCAACCTCCTGGTCGGAGAAGATGGGCACATCAA GATCGCTGACTTTGGTGTGAGCAATGAATTCAAGGGCAGTGACGCGCTCCTCTCCAACACCGTGGGCACG CCCGCCTTCATGGCACCCGAGTCGCTCTCTGAGACCCGCAAGATCTTCTCTGGGAAGGCCTTGGATGTTT GGGCCATGGGTGTGACACTATACTGCTTTGTCTTTGGCCAGTGCCCATTCATGGACGAGCGGATCATGTG TTTACACAGTAAGATCAAGAGTCAGGCCCTGGAATTTCCAGACCAGCCCGACATAGCTGAGGACTTGAAG GACCTGATCACCCGTATGCTGGACAAGAACCCCGAGTCGAGGATCGTGGTGCCGGAAATCAAGCTGCACC CCTGGGTCACGAGGCATGGGGCGGAGCCGTTGCCGTCGGAGGATGAGAACTGCACGCTGGTCGAAGTGAC TGAAGAGGAGGTCGAGAACTCAGTCAAACACATTCCCAGCTTGGCAACCGTGATCCTGGTGAAGACCATG ATACGTAAACGCTCCTTTGGGAACCCATTCGAGGGCAGCCGGCGGGAGGAACGCTCACTGTCAGCGCCTG GAAACTTGCTCACGAAGCAAGGCAGCGAAGACAACCTCCAGGGCACCGACCCGCCCCCCGTGGGGGAGGA GGAAGTGCTCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC219152 representing NM_153499 Red=Cloning site Green=Tags(s) MSSCVSSQPSSNRAAPQDELGGRGSSSSESQKPCEALRGLSSLSIHLGMESFIVVTECEPGCAVDLGLAR DRPLEADGQEVPLDSSGSQARPHLSGRKLSLQERSQGGLAAGGSLDMNGRCICPSLPYSPVSSPQSSPRL PRRPTVESHHVSITGMQDCVQLNQYTLKDEIGKGSYGVVKLAYNENDNTYYAMKVLSKKKLIRQAGFPRR PPPRGTRPAPGGCIQPRGPIEQVYQEIAILKKLDHPNVVKLVEVLDDPNEDHLYMVFELVNQGPVMEVPT LKPLSEDQARFYFQDLIKGIEYLHYQKIIHRDIKPSNLLVGEDGHIKIADFGVSNEFKGSDALLSNTVGT PAFMAPESLSETRKIFSGKALDVWAMGVTLYCFVFGQCPFMDERIMCLHSKIKSQALEFPDQPDIAEDLK DLITRMLDKNPESRIVVPEIKLHPWVTRHGAEPLPSEDENCTLVEVTEEEVENSVKHIPSLATVILVKTM IRKRSFGNPFEGSRREERSLSAPGNLLTKQGSEDNLQGTDPPPVGEEEVLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites1 | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_153499 |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| RefSeq Size | 5577 bp |
| RefSeq ORF | 1626 bp |
| Locus ID | 10645 |
| UniProt ID | Q96RR4 |
| Cytogenetics | 12q24.31 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase, Transcription Factors |
| Protein Pathways | Adipocytokine signaling pathway |
| Gene Summary | The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. The major isoform of this gene plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Protein products of this gene also phosphorylate AMP-activated protein kinase (AMPK). This gene has its strongest expression in the brain and influences signalling cascades involved with learning and memory, neuronal differentiation and migration, neurite outgrowth, and synapse formation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. The identified isoforms differ in their ability to undergo autophosphorylation and to phosphorylate downstream kinases. [provided by RefSeq, Jul 2012] |
| MW | 59.6 kDa |