CLK2 (NM_003993) Human Untagged Clone

CLK2 (NM_003993) Human Untagged Clone

CLK2 (NM_003993) Human Untagged Clone

Product Information

Product Name CLK2 (NM_003993) Human Untagged Clone
Cat.No CDL25143
Description CLK2 (untagged)-Kinase deficient mutant (K192M) of Human CDC-like kinase 2 (CLK2)
Type Human Untagged Clone
Tag Tag Free
Symbol CLK2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data Fully Sequenced ORF (showhide)


>OriGene ORF within SC323449 sequence for NM_003993 edited (data generated by NextGen Sequencing) ATGCCGCATCCTCGAAGGTACCACTCCTCAGAGCGAGGCAGCCGGGGGAGTTACCGTGAA CACTATCGGAGCCGAAAGCATAAGCGACGAAGAAGTCGCTCCTGGTCAAGTAGTAGTGAC CGGACACGACGGCGTCGGCGAGAGGACAGCTACCATGTCCGTTCTCGAAGCAGTTATGAT GATCGTTCGTCCGACCGGAGGGTGTATGACCGGCGATACTGTGGCAGCTACAGACGCAAC GATTATAGCCGGGATCGGGGAGATGCCTACTATGACACAGACTATCGGCATTCCTATGAA TATCAGCGGGAGAACAGCAGTTACCGCAGCCAGCGCAGCAGCCGGAGGAAGCACAGACGG CGGAGGAGGCGCAGCCGGACATTTAGCCGCTCATCTTCGCACAGCAGCCGGAGAGCCAAG AGTGTAGAGGACGACGCTGAGGGCCACCTCATCTACCACGTCGGGGACTGGCTACAAGAG CGATATGAAATCGTTAGCACCTTAGGAGAGGGGACCTTCGGCCGAGTTGTACAATGTGTT GACCATCGCAGGGGTGGGGCTCGAGTTGCCCTGATGATCATTAAGAATGTGGAGAAGTAC AAGGAAGCAGCTCGACTTGAGATCAACGTGCTAGAGAAAATCAATGAGAAAGACCCTGAC AACAAGAACCTCTGTGTCCAGATGTTTGACTGGTTTGACTACCATGGCCACATGTGTATC TCCTTTGAGCTTCTGGGCCTTAGCACCTTCGATTTCCTCAAAGACAACAACTACCTGCCC TACCCCATCCACCAAGTGCGCCACATGGCCTTCCAGCTGTGCCAGGCTGTCAAGTTCCTC CATGATAACAAGCTGACACATACAGACCTCAAGCCTGAAAATATTCTGTTTGTGAATTCA GACTATGAGCTCACCTACAACCTAGAGAAGAAGCGAGATGAGCGCAGTGTGAAGAGCACA GCTGTGCGGGTGGTAGACTTTGGCAGTGCCACCTTTGACCATGAGCACCATAGCACCATT GTCTCCACTCGCCATTACCGAGCACCAGAAGTCATCCTTGAGTTGGGCTGGTCACAGCCT TGTGATGTGTGGAGTATAGGCTGCATCATCTTTGAATACTATGTGGGATTCACCCTCTTC CAGACCCATGACAACAGAGAGCATCTAGCCATGATGGAAAGGATCTTGGGTCCTATCCCT TCCCGGATGATCCGAAAGACAAGAAAGCAGAAATATTTTTACCGGGGTCGCCTGGATTGG GATGAGAACACATCAGCTGGGCGCTATGTTCGTGAGAACTGCAAACCGCTGCGGCGGTAT CTGACCTCAGAGGCAGAGGAACACCACCAGCTCTTCGATCTGATTGAAAGCATGCTAGAG TATGAACCAGCTAAGCGGCTGACCTTGGGTGAAGCCCTTCAGCATCCTTTCTTCGCCCGC CTTCGGGCTGAGCCGCCCAACAAGTTGTGGGACTCCAGTCGGGATATCAGTCGGTGA Clone variation with respect to NM_003993.2575 a=>t





5' Read Nucleotide Sequence (showhide)


>OriGene 5' read for mutant NM_003993 unedited
CCCGCCCGCAACAGCAAAGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGT
GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGAGAGGCCAGA
GCCGGAGACCGAGCTGGGATCGGGCCCCGGGCGGGGGCGGTGCGAGCGGCGCCAAGCAGATCTTAGGGGC
GGGGACGGAGCCGGGGCGGGCGGGACTGAAGCGGAGCCCGGGAACGGGGCGGGAGGTCCCAGGGTCCCCG
GGTTGGGGGGGGTGGAGCAGGTTTAGGAATAGCAGACAGCAAAACCTTACAAGCCCAACGGATGAATTAT
AGACGAAAAAAAAACATAGCAAAAAATCCCAGGCAAAAAAATAAACAAAAAACAAGGGGGAGA






Kinase domain sequence (showhide)


>SC323449 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutationTTYGAGKKGTTRRACTTAGAGAGGGGACTTCGGCCGAGTTGTACAATGTGTTGACCATCGCAGCCTCTCC
YSYCAACTTACAGGGGTGGGGCTCGAGTTGCCCTGATGATCATTAAGAATGTGGAGAAGTACAAGGAAGC
AGCTCGACTTGAGATCAACGTGCTAGAGAAAATCAATGAGAAAGACCCTGACAACAAGAACCTCTGTGTC
CAGATGTTTGACTGGTTTGACTACCATGGCCACATGTGTATCTCC
Restriction Sites1 Please inquire
ACCN NM_003993
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
RefSeq NM_003993.2, NP_003984.2
RefSeq Size 2175 bp
RefSeq ORF 1497 bp
Locus ID 1196
UniProt ID P49760
Cytogenetics 1q22
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Gene Summary This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.