Human ALPP (PLAP) Knockout HeLa Cell Line
Product Overview
| Catalog Number | PCT0010 |
| Product Size | 1 × 106 cells/vial |
| Host Type | Human |
| Form | Cell Line |
| Tissue | Cervix |
Shipping & Storage Information
| Storage Condition | Upon arrival, the vial should be stored in liquid nitrogen vapor phase. Storage buffer: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether |
| Shipping Condition | Shipped on dry ice. |
Product Details
| Parental Cell Line | HeLa |
| Passage Number | <20 |
| Viability | ~80% |
| Mutation Description | CRISPR/Cas9 system is used to generate knockout cell lines. Homozygous: 17 bp deletion in exon 5. |
| Adherent / Suspension | Adherent |
| Cell Type | Epithelial Cells |
| Mycoplasma Free | Yes |
| Knockout Validation | Sanger Sequencing, Western Blot (WB) |
| Cell Medium | Culture medium: DMEM (High Glucose) + 10% FBS. Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose. |
| Initial Handling Guidelines | 1. Thaw the vial in 37ºC water bath approximately 1-2 minutes. 2. Transfer the cell suspension to a 15 ml/50 ml conical sterile polypropylene centrifuge tube containing pre-warmed culture medium. Wash vial with an additional culture medium to collect remaining cells, and centrifuge at 201 × g (rcf) for 5 minutes at room temperature. 3. Resuspend the cell pellet in 5 ml pre-warmed culture medium and count cell number. Seed cells in an appropriate cell culture flask at a density of 2×104 cells/cm2. This should allow for confluency within 48 hours. 4. Incubate the culture at 37ºC incubator with 5% CO2. Cultures should be monitored daily. |
| Subculture Guidelines | All seeding densities should be based on cell counts gained by established methods. A guide seeding density of 2x104 cells/cm2 is recommended for confluency (80-90% confluence) within 48 hours. A partial media change 24 hours prior to subculture may be helpful to encourage growth, if required. Cells should be passaged when they have achieved 80-90% confluence. |
| Disease | Adenocarcinoma |
| Gender | Female |
| Applications | Western blot. Predicted molecular weight: 58 kDa. |
| Description | Human wild-type HeLa cell line as the recommended control. |
Target Info
| Cellular Localization | Cell membrane. |
| Target Sequence Similarities | Belongs to the alkaline phosphatase family. |