Human CDC42BPA Knockout HeLa Cell Line

Human CDC42BPA Knockout HeLa Cell Line

Human CDC42BPA Knockout HeLa Cell Line

Product Overview

Catalog Number KCT0017
Product Size 1 × 106 cells/vial, 1 mL
Host Type Human
Form Cell Line
Tissue Cervix

Shipping & Storage Information

Storage Condition Upon arrival, the vial should be stored in liquid nitrogen vapor phase.
Storage buffer: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether
Shipping Condition Shipped on dry ice.

Product Details

Parental Cell Line HeLa
Passage Number <20
Viability ~80%
Mutation Description CRISPR/Cas9 system is used to generate knockout cell lines. Mutation: 10 bp deletion in exon 3, 37 bp insertion in exon 3, and 5 bp deletion in exon 3.
Adherent / Suspension Adherent
Cell Type Epithelial Cells
Mycoplasma Free Yes
Knockout Validation Sanger Sequencing
Cell Medium Culture medium: DMEM (High Glucose) + 10% FBS
Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Initial Handling Guidelines 1. Thaw the vial in 37ºC water bath approximately 1-2 minutes.
2. Transfer the cell suspension to a 15 ml/50 ml conical sterile polypropylene centrifuge tube containing pre-warmed culture medium. Wash vial with an additional culture medium to collect remaining cells, and centrifuge at 201 × g (rcf) for 5 minutes at room temperature.
3. Resuspend the cell pellet in 5 ml pre-warmed culture medium and count cell number. Seed cells in an appropriate cell culture flask at a density of 2×104 cells/cm2. This should allow for confluency within 48 hours.
4. Incubate the culture at 37ºC incubator with 5% CO2. Cultures should be monitored daily.
Subculture Guidelines Cells should be passaged when they have achieved 80-90% confluence. A partial media change 24 hours prior to subculture may be helpful to encourage growth.
Disease Adenocarcinoma
Gender Female
Description Human wild-type HeLa cell line as the recommended control.

Target Info

Target Function May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion.
Cellular Localization Cytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery.
Tissue Specificity Abundant in the heart, brain, skeletal muscle, kidney, and pancreas.
Little to no expression in the lung and liver.
Target Sequence Similarities Belongs to the AGC Ser/Thr protein kinase family, DMPK subfamily.