Human CKMT1A Knockout HEK293T Cell Line
|Product Size||1 × 106 cells/vial, 1 mL|
Shipping & Storage Information
|Storage Condition||Upon arrival, the vial should be stored in liquid nitrogen vapor phase.
Storage buffer: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether
|Shipping Condition||Shipped on dry ice.|
|Parental Cell Line||HEK293T|
|Mutation Description||CRISPR/Cas9 system is used to generate knockout cell lines. Homozygous: 1 bp insertion in exon 4.|
|Adherent / Suspension||Adherent|
|Cell Type||Epithelial Cells|
|Knockout Validation||Sanger Sequencing|
|Cell Medium||Culture medium: DMEM (High Glucose) + 10% FBS.
Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
|Initial Handling Guidelines||1. Thaw the vial in 37ºC water bath approximately 1-2 minutes.
2. Transfer the cell suspension to a 15 ml/50 ml conical sterile polypropylene centrifuge tube containing pre-warmed culture medium. Wash vial with an additional culture medium to collect remaining cells, and centrifuge at 201 × g (rcf) for 5 minutes at room temperature.
3. Resuspend the cell pellet in 5 ml pre-warmed culture medium and count cell number. Seed cells in an appropriate cell culture flask at a density of 2×104 cells/cm2. This should allow for confluency within 48 hours.
4. Incubate the culture at 37ºC incubator with 5% CO2. Cultures should be monitored daily.
|Subculture Guidelines||Cells should be passaged when they have achieved 80-90% confluence. A partial media change 24 hours prior to subculture may be helpful to encourage growth.|
|Description||Human wild-type HEK293T cell line as the recommended control.|
|Target Function||Creatine kinase MT is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. Overexpression of ubiquitous mitochondrial creatine kinase is involved in many malignant cancers with poor prognosis.|
|Cellular Localization||Cell membrane and mitochondrial.|
|Target Sequence Similarities||Belongs to the creatine kinase isoenzyme family.|