Human CSNK1D Knockout HeLa Cell Lysate

Human CSNK1D Knockout HeLa Cell Lysate

Human CSNK1D Knockout HeLa Cell Lysate

Product Overview

Catalog Number KCT0044
Product Size 1 kit
Host Type Human
Form Cell Lysate

Shipping & Storage Information

Storage Condition Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
Shipping Condition Shipped on dry ice.

Product Details

Parental Cell Line HeLa
Passage Number <20
Mutation Description CRISPR/Cas9 system is used to generate knockout cell lines. Mutation: 11 bp insertion in exon 1 and 1 bp deletion in exon 1.
Cell Type Epithelial Cells
Mycoplasma Free Yes
Knockout Validation Sanger Sequencing, Western Blot (WB)
Lysis Buffer RIPA buffer added with a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
Disease Adenocarcinoma
Gender Female
Applications Western blot. Predicted molecular weight: 47 kDa.
Description As WB control, the lyophilizate is recommended to be resuspend in 50 µL of LDS Sample Buffer to have a final concentration of 2 mg/ml.
For reducing conditions, a final concentration of 0.1M DTT is recommended.

Target Info

Cellular Localization Cytoplasm and nucleus.
Tissue Specificity Expressed in all tissues examined, including brain, heart, lung, liver, kidney, skeletal muscle, pancreas, and placenta.
Target Sequence Similarities Belongs to the CK1 Ser/Thr protein kinase family, Casein kinase I subfamily.
Post-Translational Modifications Autophosphorylated on serine and threonine residues.