Human DAPK1 Knockout HEK293T Cell Lysate

Storage Condition	Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
Shipping Condition	Shipped on dry ice.

Parental Cell Line	HEK293T
Passage Number	<20
Mutation Description	CRISPR/Cas9 system is used to generate knockout cell lines. Mutation: 1 bp insertion in exon3.
Cell Type	Epithelial Cells
Mycoplasma Free	Yes
Knockout Validation	Sanger Sequencing
Lysis Buffer	RIPA buffer added with a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
Description	As WB control, the lyophilizate is recommended to be resuspend in 50 µL of LDS Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, a final concentration of 0.1M DTT is recommended.

Cellular Localization	Cytoplasm. Colocalizes with the actin filament system.
Target Sequence Similarities	Belongs to the CAMK Ser/Thr protein kinase family, DAP kinase subfamily.
Post-Translational Modifications	The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome.