Human DAPK3 Knockout HEK293T Cell Line

Human DAPK3 Knockout HEK293T Cell Line

Human DAPK3 Knockout HEK293T Cell Line

Product Overview

Catalog Number KCT0024
Product Size 1 × 106 cells/vial, 1 mL
Host Type Human
Form Cell Line
Tissue Kidney

Shipping & Storage Information

Storage Condition Upon arrival, the vial should be stored in liquid nitrogen vapor phase.
Storage buffer: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether
Shipping Condition Shipped on dry ice.

Product Details

Parental Cell Line HEK293T
Passage Number <20
Viability ~80%
Mutation Description CRISPR/Cas9 system is used to generate knockout cell lines. Homozygous: 1 bp insertion in exon 1. Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest.
Adherent / Suspension Adherent
Cell Type Epithelial Cells
Mycoplasma Free Yes
Knockout Validation Sanger Sequencing
Cell Medium Culture medium: DMEM (High Glucose) + 10% FBS.
Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Initial Handling Guidelines 1. Thaw the vial in 37ºC water bath approximately 1-2 minutes.
2. Transfer the cell suspension to a 15 ml/50 ml conical sterile polypropylene centrifuge tube containing pre-warmed culture medium. Wash vial with an additional culture medium to collect remaining cells, and centrifuge at 201 × g (rcf) for 5 minutes at room temperature.
3. Resuspend the cell pellet in 5 ml pre-warmed culture medium and count cell number. Seed cells in an appropriate cell culture flask at a density of 2×104 cells/cm2. This should allow for confluency within 48 hours.
4. Incubate the culture at 37ºC incubator with 5% CO2. Cultures should be monitored daily.
Subculture Guidelines Cells should be passaged when they have achieved 80-90% confluence. A partial media change 24 hours prior to subculture may be helpful to encourage growth.
Applications Western blot. Predicted molecular weight: 53 kDa.
Description Human wild-type HEK293T cell line is the recommended control.

Target Info

Cellular Localization Cytoplasm, nucleus, and PML body.
Target Sequence Similarities Belongs to the CAMK Ser/Thr protein kinase family, DAP kinase subfamily.
Post-Translational Modifications Ubiquitinated and autophosphorylated.