Human PPP1R2 Knockout HeLa Cell Line

Human PPP1R2 Knockout HeLa Cell Line

Human PPP1R2 Knockout HeLa Cell Line

Product Overview

Catalog Number PCT0011
Product Size 1 × 106 cells/vial
Host Type Human
Form Cell Line
Tissue Cervix

Shipping & Storage Information

Storage Condition Upon arrival, the vial should be stored in liquid nitrogen vapor phase.
Storage buffer: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether
Shipping Condition Shipped on dry ice.

Product Details

Parental Cell Line HeLa
Passage Number <20
Viability ~80%
Mutation Description CRISPR/Cas9 system is used to generate knockout cell lines. Homozygous: Insertion of the selection cassette in exon 1.
Adherent / Suspension Adherent
Cell Type Epithelial Cells
Mycoplasma Free Yes
Knockout Validation Sanger Sequencing
Cell Medium Culture medium: DMEM (High Glucose) + 10% FBS.
Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Initial Handling Guidelines 1. Thaw the vial in 37ºC water bath approximately 1-2 minutes.
2. Transfer the cell suspension to a 15 ml/50 ml conical sterile polypropylene centrifuge tube containing pre-warmed culture medium. Wash vial with an additional culture medium to collect remaining cells, and centrifuge at 201 × g (rcf) for 5 minutes at room temperature.
3. Resuspend the cell pellet in 5 ml pre-warmed culture medium and count cell number. Seed cells in an appropriate cell culture flask at a density of 2×104 cells/cm2. This should allow for confluency within 48 hours.
4. Incubate the culture at 37ºC incubator with 5% CO2. Cultures should be monitored daily.
Subculture Guidelines All seeding densities should be based on cell counts gained by established methods.
A guide seeding density of 2x104 cells/cm2 is recommended for confluency (80-90% confluence) within 48 hours.
A partial media change 24 hours prior to subculture may be helpful to encourage growth, if required.
Cells should be passaged when they have achieved 80-90% confluence.
Disease Adenocarcinoma
Gender Female
Description Human wild-type HeLa cell line as the recommended control.

Target Info

Target Function Inhibitor of protein-phosphatase 1.
Target Sequence Similarities Belongs to the protein phosphatase inhibitor 2 family.
Post-Translational Modifications Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.