Human PPP1R2 Knockout HeLa Cell Lysate

Storage Condition	Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
Shipping Condition	Shipped on dry ice.

Parental Cell Line	HeLa
Passage Number	<20
Viability	~80%
Mutation Description	CRISPR/Cas9 system is used to generate knockout cell lines. Mutation: Insertion of the selection cassette in exon1.
Adherent / Suspension	Adherent
Cell Type	Epithelial Cells
Mycoplasma Free	Yes
Knockout Validation	Sanger Sequencing
Lysis Buffer	RIPA buffer added with a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
Disease	Adenocarcinoma
Gender	Female
Description	As WB control, the lyophilizate is recommended to be resuspend in 50 µL of LDS Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, a final concentration of 0.1M DTT is recommended.

Target Function	Inhibitor of protein-phosphatase 1.
Target Sequence Similarities	Belongs to the protein phosphatase inhibitor 2 family.
Post-Translational Modifications	Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.