MAP3K4 (NM_005922) Human Tagged ORF Clone

MAP3K4 (NM_005922) Human Tagged ORF Clone

MAP3K4 (NM_005922) Human Tagged ORF Clone

Product Information

Product Name MAP3K4 (NM_005922) Human Tagged ORF Clone
Cat.No CDEP1003
Description MAP3K4 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant 1
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MAP3K4
Synonyms MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites1 SgfI-MluI
    


Cloning Scheme for this gene
    


Plasmid Map
ACCN NM_005922
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
RefSeq NM_005922.4
RefSeq Size 5564 bp
RefSeq ORF 4827 bp
Locus ID 4216
UniProt ID Q9Y6R4
Cytogenetics 6q26
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways GnRH signaling pathway, MAPK signaling pathway
Gene Summary The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, May 2014]
MW 181.6 kDa