PKC gamma (PRKCG) (NM_002739) Human Untagged Clone
Product Information
| Product Name | PKC gamma (PRKCG) (NM_002739) Human Untagged Clone |
| Cat.No | CDL25138 |
| Description | PRKCG (untagged)-Kinase deficient mutant (K380M) of Human protein kinase C, gamma (PRKCG) |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Symbol | PRKCG |
| Synonyms | PKC-gamma; PKCC; PKCG; PKCgamma; PKCI(3); SCA14 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | Fully Sequenced ORF (showhide) >OriGene ORF within SC323469 sequence for NM_002739 edited (data generated by NextGen Sequencing) ATGGCTGGTCTGGGCCCCGGCGTAGGCGATTCAGAGGGGGGACCCCGGCCCCTGTTTTGC AGAAAGGGGGCTCTGAGGCAGAAGGTGGTCCACGAAGTCAAGAGCCACAAGTTCACCGCT CGCTTCTTCAAGCAGCCCACCTTCTGCAGCCACTGCACCGACTTCATCTGGGGTATCGGA AAGCAGGGCCTGCAATGTCAAGTCTGCAGCTTTGTGGTTCATCGACGATGCCACGAATTT GTGACCTTCGAGTGTCCAGGCGCTGGGAAGGGCCCCCAGACGGACGACCCCCGGAACAAA CACAAGTTCCGCCTGCATAGCTACAGCAGCCCCACCTTCTGCGACCACTGTGGCTCCCTC CTCTACGGGCTTGTGCACCAGGGCATGAAATGCTCCTGCTGCGAGATGAACGTGCACCGG CGCTGTGTGCGTAGCGTGCCCTCCCTGTGCGGTGTGGACCACACCGAGCGCCGCGGGCGC CTGCAGCTGGAGATCCGGGCTCCCACAGCAGATGAGATCCACGTAACTGTTGGCGAGGCC CGTAACCTAATTCCTATGGACCCCAACGGTCTCTCTGATCCCTATGTGAAACTGAAGCTC ATCCCAGACCCTCGGAACCTGACGAAACAGAAGACCCGAACGGTGAAAGCCACGCTAAAC CCTGTGTGGAATGAGACCTTTGTGTTCAACCTGAAGCCAGGGGATGTGGAGCGCCGGCTC AGCGTGGAGGTGTGGGACTGGGACCGGACCTCCCGCAACGACTTCATGGGGGCCATGTCC TTTGGCGTCTCGGAGCTGCTCAAGGCGCCCGTGGATGGCTGGTACAAGTTACTGAACCAG GAGGAGGGCGAGTATTACAATGTGCCGGTGGCCGATGCTGACAACTGCAGCCTCCTCCAG AAGTTTGAGGCTTGTAACTACCCCCTGGAATTGTATGAGCGGGTGCGGATGGGCCCCTCT TCCTCTCCCATCCCCTCCCCTTCCCCTAGTCCCACCGACCCCAAGCGCTGCTTCTTCGGG GCGAGTCCAGGACGCCTGCACATCTCCGACTTCAGCTTCCTCATGGTTCTAGGAAAAGGC AGTTTTGGGAAGGTGATGCTGGCCGAGCGCAGGGGCTCTGATGAGCTCTACGCCATCATG ATCTTGAAAAAGGACGTGATCGTCCAGGACGACGATGTGGACTGCACGCTGGTGGAGAAA CGTGTGCTGGCGCTGGGGGGCCGGGGTCCTGGCGGCCGGCCCCACTTCCTCACCCAGCTC CACTCCACCTTCCAGACCCCGGACCGCCTGTATTTCGTGATGGAGTACGTCACCGGGGGA GACTTGATGTACCACATTCAACAGCTGGGCAAGTTTAAGGAGCCCCATGCAGCGTTCTAC GCGGCAGAAATCGCTATCGGCCTCTTCTTCCTTCACAATCAGGGCATCATCTACAGGGAC CTGAAGCTGGACAATGTGATGCTGGATGCTGAGGGACACATCAAGATCACTGACTTTGGC ATGTGTAAGGAGAACGTCTTCCCCGGGACGACAACCCGCACCTTCTGCGGGACCCCGGAC TACATAGCCCCGGAGATCATTGCCTACCAGCCCTATGGGAAGTCTGTCGATTGGTGGTCC TTTGGAGTTCTGCTGTATGAGATGTTGGCAGGACAGCCTCCCTTCGATGGGGAGGACGAG GAGGAGCTGTTTCAGGCCATCATGGAACAAACTGTCACCTACCCCAAGTCGCTTTCCCGG GAAGCCGTGGCCATCTGCAAGGGGTTCCTGACCAAGCACCCAGGGAAGCGCCTGGGCTCA GGGCCTGATGGGGAACCTACCATCCGTGCACATGGCTTTTTCCGCTGGATTGACTGGGAG CGGCTGGAACGATTGGAGATCCCGCCTCCTTTCAGACCCCGCCCGTGTGGCCGCAGCGGC GAGAACTTTGACAAGTTCTTCACGCGGGCGGCGCCAGCGCTGACCCCTCCAGACCGCCTA GTCCTGGCCAGCATCGACCAGGCCGATTTCCAGGGCTTCACCTACGTGAACCCCGACTTC GTGCACCCGGATGCCCGCAGCCCCACCAGCCCAGTGCCTGTGCCCGTCATGTAA Clone variation with respect to NM_002739.372 c=>t;567 t=>c;1139 a=>t 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for mutant NM_002739 unedited ACCGCCGTATCAGCAACTGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGG TGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCCCTGGCGGA GCCGGCGCGCCCGGGGTGCCGCTCCCTGCCTGGCGCGCTCCGCACCTGGAGGTGCCTTGCCCCTCTCCTG CCCACCTCGGAATTTCCCTGTGGCTCCTTTGATCCTTCGAGTCTCCAGCTCCTCTCCCTTCCACCTGTTT CCCCCAAGAAAGGCAGGATCCTGGTCCCTGCTACGTTTCTGGGGGCCATGGCTGGTCTGGGCCCCGGCGT AGGCGATTCAGAGGGGGGGACCCCCGGCCCCTGTTTTGCAAAAAGGGGGCTCTGAAGGCAGAAAGGTGGT CCACAAAGTCAAGAACCCACAATTTCACCGCCTCGCTTCTTCAACCACCCCACCTTTCTGCAGCACCTGG ACCGACTTCTTCTGGGGTATCGAAAGGCAGGGCCCGGCATGGTCAAGTCTGACGCTTTGGGGTTAATCAC CAATGCCACGAATTTTTGACCTTCATGTGTCCAGCCCTGGAAAGGGCCCCCAAACGGAAACCCCGGAACA AACAAGTTTCCCTCTGTATATCACAGACCACCTTTGTGACACAGGGGGCTCCCTCTACGGGTTGGCCAAG GCTAGAAATCTCTGCGGATATAATGTCACGCCTTTGCATCAGGCCCTCGTCGCGTGGCCACACGAGCCCG CGCCCGGGCGGGAGTATGGGGGCGCACAAAGT Kinase domain sequence (showhide) >SC323469 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutationTYTYMKGGATCTAGGAAGGCAGTTTTGGGAGGTGATGCTGGCCGAGCGCAGGGGCTCTGATGAGCTCTAC GCCATCATGATCTTGAAAAAGGACGTGATCGTCCAGGACGACGATGTGGACTGCACGCTGGTGGAGAAAC GTGTGCTGGCGCTGGGGGGCCGGGGTCCTGGCGGCCGGCCCCACTTCCTCACCCAGCTCCACTCCACCTT CCAGACCCCGGACCGCCTGTATTTCGTGATGGAGTACGTCACCGG |
| Restriction Sites1 | Please inquire |
| ACCN | NM_002739 |
| Insert Size | 3500 bp |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| RefSeq | NM_002739.3, NP_002730.1 |
| RefSeq Size | 3143 bp |
| RefSeq ORF | 2094 bp |
| Locus ID | 5582 |
| UniProt ID | P05129 |
| Cytogenetics | 19q13.42 |
| Domains | C2, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Calcium signaling pathway, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, Leukocyte transendothelial migration, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, Tight junction, Vascular smooth muscle contraction, VEGF signaling pathway, Vibrio cholerae infection, Wnt signaling pathway |
| Gene Summary | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. |