SLK (NM_014720) Human Tagged ORF Clone
Product Information
| Product Name | SLK (NM_014720) Human Tagged ORF Clone |
| Cat.No | CDEP1009 |
| Description | SLK (Myc-DDK-tagged)-Human STE20-like kinase (SLK) |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | SLK |
| Synonyms | bA16H23.1; LOSK; se20-9; STK2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Sequence Data | ORF Nucleotide Sequence (showhide) >RC220128 representing NM_014720 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCCTTCTTCAATTTCCGTAAGATCTTCAAGTTGGGGAGCGAGAAGAAGAAGAAGCAGTACGAACACG TGAAGAGGGACCTGAACCCCGAAGACTTTTGGGAGATTATAGGAGAACTGGGCGACGGAGCCTTTGGGAA AGTGTACAAGGCCCAGAATAAAGAGACCAGTGTTTTAGCTGCTGCAAAAGTGATTGACACTAAATCTGAA GAAGAACTTGAAGATTACATGGTAGAGATTGACATATTAGCATCTTGTGATCACCCAAATATAGTCAAGC TTCTAGATGCCTTCTATTATGAGAACAATCTTTGGATCCTCATTGAATTTTGTGCAGGTGGAGCAGTAGA TGCTGTGATGCTTGAACTTGAGAGACCATTAACTGAGTCCCAAATACAAGTAGTTTGCAAGCAGACTTTA GATGCATTGAACTACTTACATGATAATAAGATCATCCACAGAGATCTGAAGGCTGGCAACATTCTCTTTA CCTTAGATGGAGATATCAAATTGGCGGATTTTGGAGTATCAGCTAAAAACACGAGGACAATTCAAAGAAG AGATTCCTTTATTGGTACACCATATTGGATGGCTCCTGAAGTAGTCATGTGTGAAACATCTAAGGACAGA CCCTATGACTACAAAGCTGATGTTTGGTCCCTGGGTATCACTTTAATAGAAATGGCTGAGATAGAACCAC CTCATCATGAATTAAATCCAATGCGAGTGCTGCTAAAAATAGCAAAATCTGAGCCACCTACATTAGCACA GCCATCCAGATGGTCTTCAAATTTTAAGGACTTTCTAAAGAAATGCTTAGAAAAGAATGTGGATGCCAGG TGGACTACATCTCAGCTGCTGCAGCATCCCTTTGTTACTGTTGATTCCAACAAACCCATCCGAGAATTGA TTGCAGAGGCGAAGGCTGAAGTAACAGAAGAAGTTGAAGATGGCAAAGAGGAAGATGAAGAGGAGGAAAC AGAAAATTCTCTGCCAATACCTGCAAGTAAGCGTGCATCTTCTGACCTTAGTATCGCCAGCTCTGAAGAA GATAAACTTTCACAAAATGCTTGTATTTTGGAGTCTGTCTCAGAAAAAACAGAACGTAGTAACTCTGAAG ATAAACTCAACAGCAAAATTCTTAATGAAAAACCCACCACTGATGAACCTGAAAAGGCTGTGGAGGATAT TAATGAACATATTACCGATGCTCAGTTAGAAGCAATGACTGAACTCCATGACAGAACAGCAGTAATCAAG GAGAATGAAAGAGAGAAGAGGCCCAAGCTTGAAAATCTGCCTGACACAGAAGACCAAGAAACTGTGGACA TTAATTCAGTCAGTGAAGGAAAAGAGAATAATATAATGATAACCTTAGAAACAAATATTGAACATAATCT AAAATCTGAGGAAGAAAAGGATCAGGAAAAGCAACAGATGTTTGAAAATAAGCTTATAAAATCTGAAGAA ATTAAAGATACTATTTTGCAAACAGTAGATTTAGTTTCTCAAGAGACTGGAGAAAAAGAGGCAAATATTC AGGCAGTTGATAGTGAAGTTGGGCTTACAAAGGAAGACACCCAAGAGAAATTGGGGGAAGACGACAAAAC TCAAAAAGATGTGATCAGCAATACAAGTGATGTGATAGGAACATGTGAGGCAGCAGATGTGGCTCAGAAA GTGGATGAAGACAGTGCTGAGGATACGCAGAGTAATGATGGGAAAGAAGTGGTCGAAGTAGGCCAGAAAT TAATTAATAAGCCCATGGTGGGTCCTGAGGCTGGTGGTACTAAGGAAGTTCCTATTAAAGAAATAGTTGA AATGAATGAAATAGAAGAAGGTAAAAATAAGGAACAAGCAATAAACAGTTCAGAGAACATAATGGACATC AATGAGGAACCAGGAACAACTGAAGGTGAAGAAATCACTGAGTCAAGTAGCACTGAAGAAATGGAGGTCA GAAGTGTGGTGGCTGATACTGACCAAAAGGCTTTAGGAAGTGAAGTTCAGGATGCTTCTAAAGTCACTAC TCAGATAGATAAAGAGAAAAAAGAAATTCCAGTGTCAATTAAAAAAGAGCCTGAAGTTACTGTAGTTTCA CAGCCCACTGAACCTCAGCCTGTTCTAATACCCAGTATTAATATCAACTCTGACAGTGGAGAAAATAAAG AAGAAATAGGTTCTTTATCAAAAACTGAAACTATTCTGCCACCAGAATCTGAGAATCCAAAGGAAAATGA TAATGATTCAGGCACTGGTTCCACTGCTGATACTAGCAGTATTGACTTGAATTTATCCATCTCTAGCTTT CTAAGTAAAACTAAAGACAGTGGATCGATATCTTTACAAGAAACAAGAAGACAAAAGAAAACATTGAAGA AAACACGCAAATTTATTGTTGATGGTGTAGAAGTGAGTGTAACAACATCAAAGATAGTTACAGATAGTGA TTCCAAAACTGAAGAATTGCGGTTTCTTAGACGTCAGGAACTTCGGGAATTAAGATTTCTTCAGAAAGAA GAGCAAAGAGCCCAACAACAGCTCAATAGCAAACTACAGCAACAACGAGAACAAATTTTCCGGCGCTTTG AGCAGGAAATGATGAGTAAAAAGCGACAATATGACCAGGAAATTGAGAATCTAGAAAAACAGCAGAAACA GACTATCGAACGCCTGGAACAAGAGCACACAAATCGCTTGCGAGATGAAGCCAAACGCATCAAAGGAGAA CAAGAGAAAGAGTTGTCCAAATTTCAGAATATGCTGAAGAACCGAAAGAAGGAGGTTATAAATGAAGTGG AGAAAGCACCCAAAGAGCTGAGAAAAGAGCTCATGAAACGCAGGAAAGAGGAGCTTGCACAAAGCCAGCA TGCTCAGGAACAAGAGTTTGTTCAGAAACAACAGCAAGAATTAGATGGCTCTCTGAAAAAGATCATCCAG CAGCAGAAGGCAGAGTTAGCTAATATTGAGAGAGAGTGCCTGAATAACAAGCAACAGCTCATGAGAGCTC GAGAAGCTGCAATTTGGGAGCTCGAAGAACGACACTTACAAGAAAAACACCAGCTGCTCAAACAGCAGCT TAAAGATCAGTATTTCATGCAAAGACATCAGCTACTTAAGCGCCACGAGAAGGAAACAGAGCAAATGCAG CGTTACAATCAAAGACTTATTGAGGAATTGAAAAACAGACAGACTCAAGAAAGAGCAAGACTGCCCAAGA TTCAGCGCAGTGAAGCCAAGACTCGAATGGCCATGTTTAAGAAGAGTTTGAGAATTAACTCAACAGCCAC ACCAGATCAGGACCGTGATAAAATTAAACAGTTTGCTGCACAAGAAGAAAAGAGGCAGAAAAATGAGAGA ATGGCTCAGCATCAGAAACATGAGAATCAAATGCGAGATCTTCAGTTGCAGTGTGAAGCCAATGTCCGCG AACTGCATCAGCTGCAGAATGAAAAATGCCACTTGTTGGTTGAGCATGAGACTCAGAAACTGAAGGAGTT AGATGAGGAACATAGCCAAGAATTAAAGGAGTGGAGAGAGAAATTGAGACCTAGGAAAAAGACACTGGAA GAAGAGTTTGCCAGGAAACTACAGGAACAGGAAGTATTCTTTAAAATGACTGGGGAGTCTGAATGCCTTA ACCCATCAACACAGAGCCGGATTTCCAAATTTTATCCTATTCCCAGCTTGCATTCCACCGGATCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC220128 representing NM_014720 Red=Cloning site Green=Tags(s) MSFFNFRKIFKLGSEKKKKQYEHVKRDLNPEDFWEIIGELGDGAFGKVYKAQNKETSVLAAAKVIDTKSE EELEDYMVEIDILASCDHPNIVKLLDAFYYENNLWILIEFCAGGAVDAVMLELERPLTESQIQVVCKQTL DALNYLHDNKIIHRDLKAGNILFTLDGDIKLADFGVSAKNTRTIQRRDSFIGTPYWMAPEVVMCETSKDR PYDYKADVWSLGITLIEMAEIEPPHHELNPMRVLLKIAKSEPPTLAQPSRWSSNFKDFLKKCLEKNVDAR WTTSQLLQHPFVTVDSNKPIRELIAEAKAEVTEEVEDGKEEDEEEETENSLPIPASKRASSDLSIASSEE DKLSQNACILESVSEKTERSNSEDKLNSKILNEKPTTDEPEKAVEDINEHITDAQLEAMTELHDRTAVIK ENEREKRPKLENLPDTEDQETVDINSVSEGKENNIMITLETNIEHNLKSEEEKDQEKQQMFENKLIKSEE IKDTILQTVDLVSQETGEKEANIQAVDSEVGLTKEDTQEKLGEDDKTQKDVISNTSDVIGTCEAADVAQK VDEDSAEDTQSNDGKEVVEVGQKLINKPMVGPEAGGTKEVPIKEIVEMNEIEEGKNKEQAINSSENIMDI NEEPGTTEGEEITESSSTEEMEVRSVVADTDQKALGSEVQDASKVTTQIDKEKKEIPVSIKKEPEVTVVS QPTEPQPVLIPSININSDSGENKEEIGSLSKTETILPPESENPKENDNDSGTGSTADTSSIDLNLSISSF LSKTKDSGSISLQETRRQKKTLKKTRKFIVDGVEVSVTTSKIVTDSDSKTEELRFLRRQELRELRFLQKE EQRAQQQLNSKLQQQREQIFRRFEQEMMSKKRQYDQEIENLEKQQKQTIERLEQEHTNRLRDEAKRIKGE QEKELSKFQNMLKNRKKEVINEVEKAPKELRKELMKRRKEELAQSQHAQEQEFVQKQQQELDGSLKKIIQ QQKAELANIERECLNNKQQLMRAREAAIWELEERHLQEKHQLLKQQLKDQYFMQRHQLLKRHEKETEQMQ RYNQRLIEELKNRQTQERARLPKIQRSEAKTRMAMFKKSLRINSTATPDQDRDKIKQFAAQEEKRQKNER MAQHQKHENQMRDLQLQCEANVRELHQLQNEKCHLLVEHETQKLKELDEEHSQELKEWREKLRPRKKTLE EEFARKLQEQEVFFKMTGESECLNPSTQSRISKFYPIPSLHSTGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites1 | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_014720 |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| RefSeq | NM_014720.4 |
| RefSeq Size | 5629 bp |
| RefSeq ORF | 3708 bp |
| Locus ID | 9748 |
| UniProt ID | Q9H2G2 |
| Cytogenetics | 10q24.33-q25.1 |
| Domains | pkinase |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Oocyte meiosis |
| Gene Summary | Mediates apoptosis and actin stress fiber dissolution.[UniProtKB/Swiss-Prot Function] |
| MW | 142.5 kDa |