TIF1 alpha (TRIM24) (NM_003852) Human Tagged ORF Clone
Product Information
| Product Name | TIF1 alpha (TRIM24) (NM_003852) Human Tagged ORF Clone |
| Cat.No | CDEP1002 |
| Description | TRIM24 (Myc-DDK-tagged)-Human tripartite motif containing 24 (TRIM24), transcript variant 2 |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | TRIM24 |
| Synonyms | hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A; TIF1ALPHA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Sequence Data | ORF Nucleotide Sequence (showhide) >RC218319 representing NM_003852 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGTGGCGGTGGAGAAGGCGGTGGCGGCGGCGGCAGCGGCCTCGGCTGCGGCCTCCGGGGGGCCCT CGGCGGCGCCGAGCGGGGAGAACGAGGCCGAGAGTCGGCAGGGCCCGGACTCGGAGCGCGGCGGCGAGGC GGCCCGGCTCAACCTGTTGGACACTTGCGCCGTGTGCCACCAGAACATCCAGAGCCGGGCGCCCAAGCTG CTGCCCTGCCTGCACTCTTTCTGCCAGCGCTGCCTGCCCGCGCCCCAGCGCTACCTCATGCTGCCCGCGC CCATGCTGGGCTCGGCCGAGACCCCGCCACCCGTCCCTGCCCCCGGCTCGCCGGTCAGCGGCTCGTCGCC GTTCGCCACCCAAGTTGGAGTCATTCGTTGCCCAGTTTGCAGCCAAGAATGTGCAGAGAGACACATCATA GATAACTTTTTTGTGAAGGACACTACTGAGGTTCCCAGCAGTACAGTAGAAAAGTCAAATCAGGTATGTA CAAGCTGTGAGGACAACGCAGAAGCCAATGGGTTTTGTGTAGAGTGTGTTGAATGGCTCTGCAAGACGTG TATCAGAGCTCATCAGAGGGTAAAGTTCACAAAAGACCACACTGTCAGACAGAAAGAGGAAGTATCTCCA GAGGCAGTTGGTGTCACCAGCCAGCGACCAGTGTTTTGTCCTTTTCATAAAAAGGAGCAGCTGAAGCTGT ACTGTGAGACATGTGACAAACTGACATGTCGAGACTGTCAGTTGTTAGAACATAAAGAGCATAGATACCA ATTTATAGAAGAAGCTTTTCAGAATCAGAAAGTGATCATAGATACACTAATCACCAAACTGATGGAAAAA ACAAAATACATAAAATTCACAGGAAATCAGATCCAAAACAGAATTATTGAAGTAAATCAAAATCAAAAGC AGGTGGAACAGGATATTAAAGTTGCTATATTTACACTGATGGTAGAAATAAATAAAAAAGGAAAAGCTCT ACTGCATCAGTTAGAGAGCCTTGCAAAGGACCATCGCATGAAACTTATGCAACAACAACAGGAAGTGGCT GGACTCTCTAAACAATTGGAGCATGTCATGCATTTTTCTAAATGGGCAGTTTCCAGTGGCAGCAGTACAG CATTACTTTATAGCAAACGACTGATTACATACCGGTTACGGCACCTCCTTCGTGCAAGGTGTGATGCATC CCCAGTGACCAACAACACCATCCAATTTCACTGTGATCCTAGTTTCTGGGCTCAAAATATCATCAACTTA GGTTCTTTAGTAATCGAGGATAAAGAGAGCCAGCCACAAATGCCTAAGCAGAATCCTGTCGTGGAACAGA ATTCACAGCCACCAAGTGGTTTATCATCAAACCAGTTATCCAAGTTCCCAACACAGATCAGCCTAGCTCA ATTACGGCTCCAGCATATGCAGCAACAGCAACCGCCTCCACGTTTGATAAACTTTCAGAATCACAGCCCC AAACCCAATGGACCAGTTCTTCCTCCTCATCCTCAACAACTGAGATATCCACCAAACCAGAACATACCAC GACAAGCAATAAAGCCAAACCCCCTACAGATGGCTTTCTTGGCTCAACAAGCCATAAAACAGTGGCAGAT CAGCAGTGGACAGGGAACCCCATCAACTACCAACAGCACATCCTCTACTCCTTCCAGCCCCACGATTACT AGTGCAGCAGGATATGATGGAAAGGCTTTTGGTTCACCTATGATCGATTTGAGCTCACCAGTGGGAGGGT CTTATAATCTTCCCTCTCTTCCGGATATTGACTGTTCAAGTACTATTATGCTGGACAATATTGTGAGGAA AGATACTAATATAGATCATGGCCAGCCAAGACCACCCTCAAACAGAACGGTCCAGTCACCAAATTCATCA GTGCCATCTCCAGGCCTTGCAGGACCTGTTACTATGACTAGTGTACACCCCCCAATACGTTCACCTAGTG CCTCCAGCGTTGGAAGCCGAGGAAGCTCTGGCTCTTCCAGCAAACCAGCAGGAGCTGACTCTACACACAA AGTCCCAGTGGTCATGCTGGAGCCAATTCGAATAAAACAAGAAAACAGTGGACCACCGGAAAATTATGAT TTCCCTGTTGTTATAGTGAAGCAAGAATCAGATGAAGAATCTAGGCCTCAAAATGCCAATTATCCAAGAA GCATACTCACCTCCCTGCTCTTAAATAGCAGTCAGAGCTCTACTTCTGAGGAGACTGTGCTAAGATCAGA TGCCCCTGATAGTACAGGAGATCAACCTGGACTTCACCAGGACAATTCCTCAAATGGAAAGTCTGAATGG TTGGATCCTTCCCAGAAGTCACCTCTTCATGTTGGAGAGACAAGGAAAGAGGATGACCCCAATGAGGACT GGTGTGCAGTTTGTCAAAACGGAGGGGAACTCCTCTGCTGTGAAAAGTGCCCCAAAGTATTCCATCTTTC TTGTCATGTGCCCACATTGACAAATTTTCCAAGTGGAGAGTGGATTTGCACTTTCTGCCGAGACTTATCT AAACCAGAAGTTGAATATGATTGTGATGCTCCCAGTCACAACTCAGAAAAAAAGAAAACTGAAGGCCTTG TTAAGTTAACACCTATAGATAAAAGGAAGTGTGAGCGCCTACTTTTATTTCTTTACTGCCATGAAATGAG CCTGGCTTTTCAAGACCCTGTTCCTCTAACTGTGCCTGATTATTACAAAATAATTAAAAATCCAATGGAT TTGTCAACCATCAAGAAAAGACTACAAGAAGATTATTCCATGTACTCAAAACCTGAAGATTTTGTAGCTG ATTTTAGATTGATCTTTCAAAACTGTGCTGAATTCAATGAGCCTGATTCAGAAGTAGCCAATGCTGGTAT AAAACTTGAAAATTATTTTGAAGAACTTCTAAAGAACCTCTATCCAGAAAAAAGGTTTCCCAAACCAGAA TTCAGGAATGAATCAGAAGATAATAAATTTAGTGATGATTCAGATGATGACTTTGTACAGCCCCGGAAGA AACGCCTCAAAAGCATTGAAGAACGCCAGTTGCTTAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC218319 representing NM_003852 Red=Cloning site Green=Tags(s) MEVAVEKAVAAAAAASAAASGGPSAAPSGENEAESRQGPDSERGGEAARLNLLDTCAVCHQNIQSRAPKL LPCLHSFCQRCLPAPQRYLMLPAPMLGSAETPPPVPAPGSPVSGSSPFATQVGVIRCPVCSQECAERHII DNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSP EAVGVTSQRPVFCPFHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEK TKYIKFTGNQIQNRIIEVNQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVA GLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNNTIQFHCDPSFWAQNIINL GSLVIEDKESQPQMPKQNPVVEQNSQPPSGLSSNQLSKFPTQISLAQLRLQHMQQQQPPPRLINFQNHSP KPNGPVLPPHPQQLRYPPNQNIPRQAIKPNPLQMAFLAQQAIKQWQISSGQGTPSTTNSTSSTPSSPTIT SAAGYDGKAFGSPMIDLSSPVGGSYNLPSLPDIDCSSTIMLDNIVRKDTNIDHGQPRPPSNRTVQSPNSS VPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSSSKPAGADSTHKVPVVMLEPIRIKQENSGPPENYD FPVVIVKQESDEESRPQNANYPRSILTSLLLNSSQSSTSEETVLRSDAPDSTGDQPGLHQDNSSNGKSEW LDPSQKSPLHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLSCHVPTLTNFPSGEWICTFCRDLS KPEVEYDCDAPSHNSEKKKTEGLVKLTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMD LSTIKKRLQEDYSMYSKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLENYFEELLKNLYPEKRFPKPE FRNESEDNKFSDDSDDDFVQPRKKRLKSIEERQLLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites1 | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_003852 |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| RefSeq | NM_003852.4 |
| RefSeq Size | 3905 bp |
| RefSeq ORF | 3051 bp |
| Locus ID | 8805 |
| UniProt ID | O15164 |
| Cytogenetics | 7q33-q34 |
| Domains | zf-B_box, BROMO, RING, PHD, BBC |
| Protein Families | Druggable Genome, Protein Kinase, Transcription Factors |
| Gene Summary | The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| MW | 112.8 kDa |