Label-Free Ligand Binding Assays

Label-Free Ligand Binding Assays

Label-Free Ligand Binding Assays


Creative BioMart has developed a large collection of label-free ligand binding assays that allow for high-quality and efficient kinase screening & profiling to accelerate kinase research and drug discovery. With years of experience in kinase biology and drug development, we can help our customers overcome challenges and accelerate their journey to success.


The 518 protein kinases are one of the largest protein families in the human genome. Protein kinases regulate protein expression and their biological activity by catalyzing the phosphorylation of serine, threonine, or tyrosine residues. Because of their important role in most cellular signaling pathways, kinases have been recognized as major drug targets in many therapeutic areas such as oncology, cardiology, neurology, immunology, and infectious diseases. Ligand binding assays enable the systematic analysis of kinase and their small molecule ligands. Label-free detection technologies that do not require labeling of target molecules have been widely used to characterize biomolecular interactions and can be applied to drug candidate screening when combined with high-throughput capability. Label-free ligand binding assays have the advantages of highly sensitive and pathway unbiased, providing a new dimension for elucidating kinase biology and facilitating drug discovery.

Kinetic characterization of inhibitor binding to CDK2 and p38a.

Figure 1. Kinetic characterization of inhibitor binding to CDK2 and p38a. (Nordin H, et al., 2005)

Service Options

Creative BioMart offers comprehensive options for label-free ligand binding assays to meet the needs of different customers. Our scientific team will help our customers select suitable assays and develop an optimal strategic plan. If you have any additional requirements, please fill out the online inquiry form and let us know more details.

Surface Plasmon Resonance (SPR)

Surface plasmon resonance (SPR) is an optical-based technique useful for the label-free analysis of biomolecular interactions. We provide reliable, sensitive, and rapid SPR services to monitor kinase-ligand binding and obtain high-quality data of binding kinetics. We also provide SPR imaging for affinity-based biosensors to monitor binding events in real time.

Resonant Waveguide Grating (RWG)

Resonant waveguide grating (RWG) can be used to analyze biomolecular interactions by using the electromagnetic wave bound to the surface. It can meet the requirements for measuring optical enhancement. We have established a high-throughput RWG biosensor system for the exploration of potential kinase–substrate interactions.

Biolayer Interferometry (BLI)

Biolayer interferometry (BLI) is an optical analytical technique using a spectrometer to detect interference patterns formed by light reflected from an optical layer and a biolayer. We offer unparalleled BLI services to help our customers measure kinase-ligand binding and screen for kinase drug candidates.

Mass Spectrometry-Based Label-Free Binding Assays

Mass spectrometry-based label-free binding assays without the use of extrinsic labeling have been developed as complementary to labeled ligand-binding assays. The risk of label interfering with binding can be eliminated. We are equipped with advanced facilities to perform MALDI-TOF/MS and LC-MS-based assays, providing sensitive, robust, and cost-effective services.

Creative BioMart is an advanced biotech company within the field of kinase/phosphatase biology. We offer our expertise in establishing assays for kinase screening & profiling. We have state-of-the-art facilities and technology to provide efficient services with guaranteed turnaround times. Any data or details you provide will be kept strictly confidential. If you have any questions, please do not hesitate to contact us.


  • Nordin H, et al. Kinetic studies of small molecule interactions with protein kinases using biosensor technology. Analytical biochemistry, 2005, 340(2): 359-368.
For research use only. Not intended for any clinical use.