Label-Free Assays for Kinase ActivityINQUIRY
Creative BioMart offers multiple options of label-free assays to detect kinase activity, helping our customers with their kinase biology research and drug discovery. Our talented scientific team is able to provide our customers with unparalleled expertise and experience in kinase biology and pharmaceutical research.
Reversible phosphorylation and dephosphorylation are important regulatory mechanisms in both prokaryotes and eukaryotes. Kinases are responsible for catalyzing the transfer of phosphate groups from nucleoside triphosphates to acceptor groups. Their role in mediating important cellular processes and signal transduction pathways have been extensively studied. Deregulation of kinase activity has been associated with a variety of pathological conditions, such as cancer, cardiovascular diseases, and inflammation. As a result, kinases have emerged as attractive targets for drug discovery.
An effective method for monitoring kinase activity is essential for kinase research and drug development. Radiometric assays with the advantage of high sensitivity are the conventional analytical technique for kinase activity. However, these assays are time-consuming, expensive, and have a high risk of radioactive contamination. Alternative assays such as colorimetric assays and fluorescence-based assays have been developed to overcome these limitations, but they still have some drawbacks such as labor-intensive procedures, the need for labeling, and expensive instrumentation. Label-free methods have attracted increasing attention in the development of kinase assays due to their low production cost, pathway-unbiased, fast response, and high sensitivity. These assays provide a new dimension to elucidate kinase biology and facilitate drug discovery.
Figure 1. Working principle of a gold nanoparticle based indicator displacement assay. (Pezzato C, et al., 2015)
Creative BioMart offers different label-free assay formats to fulfill different needs of our customers. Our scientists will work with you to develop an optimum assay, helping you solve problems and get answers. If you can't find an assay of your interest, please fill out the online inquiry form and let us know more details. We can offer you our expertise to help you accelerate your success.
Label-Free Fluorescence Assay
We offer label-free fluorescence assay based on nanoparticles, nanoclusters, and quantum dots for direct detection of kinase activity. We have also developed aptamer-based fluorescence biosensor for targeted kinase drug discovery research.
Label-Free Electrochemical Detection
We provide a diverse range of highly sensitive label-free biosensors for electrochemical detection of different kinases. Our assays can be integrated into parallel processing biochips and are well suited for high-throughput applications.
Microfluidic Kinase Assay
We have developed microfluidic devices capable of running both on-chip and off-chip kinase assays with improved efficiency to provide our customers with high quality data within a quick turnaround time.
Creative BioMart is an advanced biotech company within the field of kinase/phosphatase biology. We offer our expertise in establishing assays for kinase screening & profiling. We have state-of-the-art facilities and technology to provide efficient services with guaranteed turnaround times. A large collection of ready-to-use kinase activity assay kits are also available to help our customers save precious time. If you have any questions, please do not hesitate to contact us.
- Pezzato C, et al. Label-free fluorescence detection of kinase activity using a gold nanoparticle based indicator displacement assay. Organic & biomolecular chemistry, 2015, 13(4): 1198-1203.
- Jia Y, et al. Current in vit