Bioluminescent Assays for Kinase ActivityINQUIRY
Creative BioMart offers high-quality services on kinase activity studies using bioluminescent assays to assist our customers in their kinase biology research and drug discovery. Our talented scientific team is able to provide our customers with unparalleled expertise and experience in kinase biology and pharmaceutical research.
Kinases, which represent important biological intersections in signaling pathways, are one of the largest and most extensively studied classes of enzymes. Kinases catalyze the largest number of post-translational modifications (PTMs) by phosphorylating a diverse array of substrates including proteins, lipids, and metabolites. Dysregulation of phosphorylation due to abnormal kinase activity can have deleterious effects and lead to serious diseases. As a corollary, kinases have been one of the most promising therapeutic targets. Many kinase-based drugs are currently undergoing development or in clinical trials.
Assays that combine the detection of biological phenomena with bioluminescence produced by luciferase have been widely used. ATP is utilized as the phosphate donor to the substrate by the majority of kinases to produce phosphorylated substrates and ADP. Most luminescence-based kinase assays use firefly luciferase, which converts the substrate luciferin into oxiluciferin in the presence of ATP, to measure the depletion of ATP. Bioluminescent assays are homogeneous and stable, offering the advantages of sensitivity, broad linearity, and robustness for high-throughput screening (HTS) applications. A number of HTS assays have been developed to measure the products of the kinase reaction.
Figure 1. Schematic representation of the bioluminescent ATP consumption assay. (Pommereau A, et al., 2004)
Creative BioMart offers custom bioluminescent assays for kinase activity based on different methods to meet the specific needs of our customers. Our scientists will work with you to develop an optimum assay. If you can’t find an assay of your interest, please fill out the online inquiry form and let us know more details. We can offer you our expertise to help you accelerate your success.
ATP Bioluminescence Assays
ATP bioluminescence assay monitoring the amount of ATP has the advantages of simple, highly sensitive, rapid, and cost effective. We provide ATP bioluminescence assays for functional studies of kinases. Our assays can be used for any kinase / substrate combination and performed in multi-well plates for HTS applications.
Luminescent ADP Detection Assays
Luminescent ADP detection assays are viable generic alternative to ATP bioluminescence assays for monitoring kinase activity. We have developed universal luminescent ADP detection assays with high sensitivity, suitable for the detection of kinase activity using a wide variety of substrates.
Continuous Luminescence Assays
Real-time monitoring of kinase activity is of fundamental importance to elucidate enzyme mechanisms and to discover new inhibitors and activators. We provide continuous luminescence assays to dynamically track the change in ADP/ATP ratio during kinase reactions.
Creative BioMart is an advanced biotech company within the field of kinase/phosphatase biology. We offer our expertise in establishing assays for kinase screening & profiling. We have state-of-the-art facilities and technology to provide efficient services with guaranteed turnaround times. A large collection of ready-to-use kinase activity assay kits are also available to help our customers save precious time. If you have any questions, please do not hesitate to contact us.
- Pommereau A, et al. Two simple and generic antibody-independent kinase assays: comparison of a bioluminescent and a microfluidic assay format. Journal of biomolecular screening, 2004, 9(5): 409-416.
- Tanega C, et al. Comparison of bioluminescent kinase assays using substrate depletion and product formation. Assay and drug development technologies, 2009, 7(6): 606-614.