Gene Mutagenesis for Phosphorylation ResearchINQUIRY
Creative BioMart provides professional gene mutagenesis services for kinase/phosphatase biology research and pharmaceutical development. Our expertise enables us to offer you complete services including single or multiple point mutations, deletions, insertions, or random mutagenesis of any given target gene.
Mutagenesis refers to the process of spontaneous or induced genetic mutation. With the development of molecular biology, gene mutagenesis has become an important technique used to change DNA sequences at the genetic level. Gene mutations are permanent and heritable changes in genetic materials, which can cause changes in protein function and affect phenotypes. Gene mutations have varying effects on health. Several types of gene mutations can cause genetic disorders.
Kinases are one of the largest and most functionally diverse gene families in humans. Kinase and phosphatases work together to actively drive signal response and act as key regulators of cellular function. Cells normally maintain basal kinase and phosphatase activity. Intracellular signaling pathways also revolve around kinases and phosphatases. Therefore, mutations in kinases and phosphatases may lead to the dysregulation of protein function in cells. Their dysfunction associated with pathogenesis makes them potential therapeutic targets. Various aspects of research using mutagenesis on kinases/phosphatases are of great significance for researchers to deeply understand signaling pathways and develop targeted therapies.
Figure 1. Multiple applications of mutagenesis in studies on CK2. (Sajnaga E, et al., 2013)
We provide comprehensive gene mutagenesis services for different research purposes. Our experts are dedicated to helping you find answers for your research and accelerate your project lifecycle. Our available services include, but are not limited to the following:
Site-directed mutagenesis (SDM) is an important technique to alter a gene or vector sequence at a selected location. We guarantee 100% accuracy of our SDM services including insertions, deletions, and point mutations. Mutant clones are applicable for any downstream application.
Chemical mutagens, error-prone PCR, saturation mutagenesis, or mutator strains are used to introduce point mutations at random positions. Randomized DNA can be cloned into the specific vector of your choice.
Mutant Library Construction
With strong expertise and state-of-the-art technology, we also provide mutant library services and downstream screening assays upon request. Multiple types of mutant libraries, such as site-directed mutagenesis libraries, random mutagenesis libraries, and precision mutant libraries, are available at Creative BioMart for your choice.
Custom Project Workflow
Features and Benefits
- Quality assurance – Strict quality control to ensure high-quality and high-purity.
- Expertise – A professional team of molecular biologists with extensive experience.
- Strong versatility – Different types of mutagenesis to meet specific needs.
- Fast turnaround time – Efficient services with guaranteed turnaround time.
- Economical pricing – Discounts available and no hidden charges.
Creative BioMart has always been committed to providing customers with high-quality products and services. Our innovative techniques coupled with our large collection of products allow us to tackle your project to save your time and effort. Simply provide us your project details and exact specifications, our scientific team will do the rest and provide satisfactory results.
If you need any further information or have any specific requirements, please do not hesitate to contact us. We sincerely look forward to further cooperation.
- Sajnaga E, et al. Site-Directed Mutagenesis in the Research of Protein Kinases-The Case of Protein Kinase CK2. Genetic Manipulation of DNA and Protein-Examples from Current Research. IntechOpen, 2013.
- Mighell T L, et al. A saturation mutagenesis approach to understanding PTEN lipid phosphatase activity and genotype-phenotype relationships. The American Journal of Human Genetics, 2018, 102(5): 943-955.